Triplex signal amplification for electrochemical DNA biosensing by coupling probe-gold nanoparticles-graphene modified electrode with enzyme functionalized carbon sphere as tracer
Triplex signal amplification for electrochemical DNA biosensing by coupling probe-gold nanoparticles-graphene modified electrode with enzyme functionalized carbon sphere as tracer
复制标题
通过耦合探针-金纳米粒子-石墨烯修饰电极与酶功能化碳球作为示踪剂进行电化学 DNA 生物传感的三重信号放大
DOI：
10.1016/j.bios.2012.01.006
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发表时间：
2012-03-15
影响因子：
12.6
通讯作者：
Yan, Feng
中科院分区：
文献类型：
--
作者：
Dong, Haifeng;Zhu, Zhu;Yan, Feng
将巯基标记的DNA链（d（GT）（29）SH）组装在电化学还原氧化石墨烯（ERGO）修饰电极上，并与辣根过氧化物酶（HRP）功能化碳球（CNS）偶联，构建了一种超灵敏的DNA电化学生物传感器。在生物传感器表面形成的异质纳米结构表现出相对良好的导体，用于加速电子传递，而HRP标记的CNS为电化学生物传感提供双重信号放大。三重信号放大策略产生了对DNA的超灵敏电化学检测，其线性范围为5个数量级（从1 × 10（-17）M到1 × 10（-13）M），低至阿摩尔水平（5 aM），并且表现出区分DNA的单碱基错配和三碱基错配序列的高选择性。该方法具有灵敏度高、特异性强、操作简便等优点，在生物分析和生物医学领域具有广阔的应用前景。(C)2012 Elsevier B.V.保留所有权利。
An ultrasensitive electrochemical DNA biosensor was constructed by assembling probe labeled gold nanoparticles (ssDNA-AuNP) on electrochemically reduced graphene oxide (ERGO) modified electrode with thiol group tagged (GT) DNA strand (d(GT)(29)SH) and coupling with horseradish peroxidase (HRP) functionalized carbon sphere (CNS) as tracer. The heteronanostructure formed on the biosensor surface appeared relatively good conductor for accelerating the electron transfer, while the HRP tagged CNS provided dual signal amplification for electrochemical biosensing. The triplex signal amplification strategy produced an ultrasensitive electrochemical detection of DNA down to attomolar level (5 aM) with a linear range of 5 orders of magnitude (from 1 x 10(-17) M to 1 x 10(-13) M), and appeared high selectivity to differentiate single-base mismatched and three-base mismatched sequences of DNA. The proposed approach provided a simple and reliable method for DNA detection with high sensitivity and specificity, indicating promising application in bioanalysis and biomedicine. (C) 2012 Elsevier B.V. All rights reserved.