Comparison of peroxidases in the retina, choroid and red blood cells.
Comparison of peroxidases in the retina, choroid and red blood cells.
复制标题
视网膜、脉络膜和红细胞中过氧化物酶的比较。
DOI：
10.1159/000267476
复制
发表时间：
1994
影响因子：
2.1
通讯作者：
Glickman,RD
中科院分区：
文献类型：
--
作者：
Wang,L;Lam,KW;Lam,TT;Glickman,RD
牛视网膜中的大多数过氧化物酶活性是谷胱甘肽（GSH）特异性的，而脉络膜含有GSH过氧化物酶和抗坏血酸过氧化物酶。在阳离子交换柱上，GSH过氧化物酶与抗坏血酸过氧化物酶明显分离。血红素蛋白的非特异性过氧化物酶活性解释了抗坏血酸氧化检测到的过氧化物酶活性。所有脉络膜血红素蛋白都含有与血红蛋白非常相似的亚单位。抗坏血酸过氧化物酶在视网膜中的缺乏表明抗坏血酸对抗光损伤的保护作用不是由于其与视网膜过氧化物酶的反应。因此，脉络膜中抗坏血酸过氧化物酶活性对H2O2的清除可能是研究中的一个重要因素，其中血清抗坏血酸浓度被人为升高至远高于正常水平，同时视网膜抗坏血酸浓度升高非常小。
Most of the peroxidase activity in the bovine retina is specific to glutathione (GSH) while the choroid contains both GSH peroxidases and ascorbate peroxidase. The GSH peroxidase was clearly separated from ascorbate peroxidase on a cation exchange column. The nonspecific peroxidase activity of hemoproteins accounts for the peroxidase activity detected by ascorbate oxidation. All choroidal hemoproteins contain sub-units very similar to those of hemoglobin. The absence of ascorbate peroxidase in the retina indicates that the protective effect of ascorbate against photic injury is not due to its reaction with retinal peroxidase. Therefore removal of H2O2by ascorbate peroxidase activity in the choroid could be a significant factor in studies where serum ascorbate concentrations are artificially raised far above the normal level concurrent to a very small rise in retinal ascorbate concentration.