Compartmentalization of the chick cerebellar cortex based on the link between the striped expression pattern of aldolase C and the topographic olivocerebellar projection
Compartmentalization of the chick cerebellar cortex based on the link between the striped expression pattern of aldolase C and the topographic olivocerebellar projection
复制标题
基于醛缩酶 C 条纹表达模式与橄榄小脑地形投影之间联系的鸡小脑皮质区室化
DOI：
10.1002/cne.23769
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发表时间：
2015
期刊：
影响因子：
2.5
通讯作者：
Sugihara
中科院分区：
文献类型：
--
作者：
Vibulyaseck S;Luo Y;Fujita H;Oh-Nishi A;Ohki-Hamazaki H;Sugihara
鸟类小脑组织成多个纵向条纹，由浦肯野细胞中醛缩酶C （zebrin II）的表达谱定义。醛缩酶C条纹模式与橄榄小脑投影模式之间的关系对于理解小脑功能区隔是至关重要的。我们用序列切片比对分析方法鉴定了所有小叶上的醛缩酶C条纹，然后通过鸡小脑内左小脑轴突的顺行和逆行标记来观察这种关系。醛缩酶C条纹从1 ~ 7小叶到8 ~ ixb小叶内侧大致一致连续。下橄榄的背侧和腹侧板（DL， VL）在这些区域以简单的中外侧地形关系投射到条纹上。第六、八小叶侧缘可见少量醛缩酶C条纹。IXa-IXb小叶和IXc-X小叶侧部多见条纹。下橄榄叶内侧柱（MC）投射到小叶VIII-X的条纹，包括增加的侧条纹，具有复杂的地形关系。醛缩酶C阳性和阴性条纹之间的明显边界通常伴随着浦肯野细胞层的间隙和轴突在地形上不同的群。尽管小鸡小脑的隔室组织与哺乳动物小脑相当，但在组织上的几个显著差异表明，哺乳动物和鸟类小脑的部分进化谱系是分开的。我们提出吻侧小叶可能是由鸟类小脑尾侧小叶的内侧条纹向吻侧延伸而进化而来。J. Comp. Neurol. 523:1886-1912, 2015©2015 Wiley Periodicals, Inc.。
The avian cerebellum is organized into multiple longitudinal stripes defined by expression profiles of aldolase C (zebrin II) in Purkinje cells. The relationship between the aldolase C striped pattern and the olivocerebellar projection pattern is crucial in understanding cerebellar functional compartmentalization. We identified all aldolase C stripes across all lobules with the serial section alignment analysis method and then looked at this relationship by anterograde and retrograde labeling of olivocerebellar axons in the chick cerebellum. Aldolase C stripes were generally consistent and continuous from lobule I through VII and to the medial part of lobules VIII–IXb. The dorsal and ventral lamellas (DL, VL) of the inferior olive projected to the stripes in these areas with a simple mediolateral topographic relation. A few aldolase C stripes appeared at the lateral edge of lobules VI–VIII. Several more stripes were added in the lateral parts of lobules IXa–IXb and IXc–X. The medial column (MC) of the inferior olive projected to the stripes in lobules VIII–X, including the added lateral stripes, with a complex topographic relation. Sharp boundaries between aldolase C‐positive and ‐negative stripes often accompanied a gap in the Purkinje cell layer and bordered topographically distinct groups of axons. Although the compartmental organization of the chick cerebellum is comparable to that of the mammalian cerebellum, several significant differences in the organization suggest partly separate evolutionary lineages of the mammalian and avian cerebella. We propose that rostral lobules may be evolved by rostral extension of medial stripes from caudal lobules in the avian cerebellum. J. Comp. Neurol. 523:1886–1912, 2015 © 2015 Wiley Periodicals, Inc.