Molecular cloning and characterization of a novel DNA virus (TTV) associated with posttransfusion hepatitis of unknown etiology
Molecular cloning and characterization of a novel DNA virus (TTV) associated with posttransfusion hepatitis of unknown etiology
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DOI：
10.1016/s1386-6346(97)00123-x
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发表时间：
1998-01-01
影响因子：
4.2
通讯作者：
Mayumi, M
中科院分区：
文献类型：
--
作者：
Okamoto, H;Nishizawa, T;Mayumi, M
从一位转氨酶升高但无已知肝炎病毒血清学标志的献血员血浆中克隆了一种新病毒TT病毒（TTV）的基因组DNA，并测定了其3739个碱基的序列。TTV在蔗糖中的密度为1.26 g/cm（3），用吐温80处理后没有变化。病毒基因组对DNA酶I和绿豆核酸酶敏感。因此，TTV是一种无包膜的单链DNA病毒。在不同的阅读框中鉴定了两个可能的开放阅读框，分别能够编码770和202个氨基酸。对来自78份献血员和肝炎患者血清的TTV分离株的356个碱基的部分序列进行比较，结果表明，TTV分离株之间的差异高达30%。在TTV DNA两个高度保守的区域设计引物，应用聚合酶链反应（PCR）技术检测血清和肝组织中TTV DNA。19例重型肝炎患者中有9例（47%）检出TTVDNA，90例慢性不明原因肝病患者中有41例（46%）检出TTVDNA。5例患者肝组织中均检出TTV DNA。其滴度等于或高于相应血清的10-100倍。这些结果表明TTV可能与一部分病因不明的急慢性肝病有关。(C)1998爱思唯尔科学爱尔兰有限公司
The genomic DNA of a novel virus named TT virus (TTV), associated with posttransfusion hepatitis of unknown etiology, was cloned from plasma of a blood donor with an elevated transaminase level but without serological markers of known hepatitis viruses, and its sequence of 3739 bases was determined. TTV had a density of 1.26 g/cm(3) in sucrose, which did not change after the treatment with Tween 80. The viral genome was sensitive to DNase I and Mung Bean Nuclease. Hence, TTV would be an unenveloped, single-stranded DNA virus. Two possible open reading frames in different frames were identified, capable of encoding 770 and 202 amino acids, respectively. When a partial sequence of 356 bases was compared among TTV isolates from 78 sera from blood donors and hepatitis patients, it showed considerable divergence with differences of up to 30%. Oligonucleotide primers were designed on two well-conserved regions for the detection of TTV DNA in serum and biopsied liver tissues by polymerase chain reaction. TTV DNA was detected in sera from 9 of 19 (47%) patients with fulminant hepatitis and 41 of 90 (46%) patients with chronic liver disease of unknown etiology. TTV DNA was detected in liver tissues of all the five patients tested. in titers equal or 10-100 times higher than those in the corresponding sera. These results indicate that TTV would be responsible for a part of acute and chronic liver disease of unknown etiology. (C) 1998 Elsevier Science Ireland Ltd.