Whole-genome sequencing of an acidophilic Rhodotorula sp. ZM1 and its phenol-degrading capability under acidic conditions
Whole-genome sequencing of an acidophilic Rhodotorula sp. ZM1 and its phenol-degrading capability under acidic conditions
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嗜酸红酵母的全基因组测序。
DOI：
10.1016/j.chemosphere.2019.05.195
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Sun F.
中科院分区：
文献类型：
--
作者：
Su X.;Zhou M.;Hu P.;Xiao Y.;Wang Z.;Mei R.;Hashmi M.Z.;Lin H.;Chen J.;Sun F.
利用全基因组测序（WGS）技术对一株嗜酸苯酚降解酵母菌进行遗传学研究，并对该菌株的生长特性、苯酚降解能力以及在极端酸性条件下的降解途径进行表征。结果表明，从酸性矿山废水（AMD）中分离到的菌株ZM 1属于担子菌酵母Rhodotorulasp.，与其他四种密切相关的红酵母属物种相比，它拥有一些独特的基因。京都基因和基因组百科全书（KEGG）分析表明，ZM 1具有降解芳香族化合物的潜力。ZM 1是一株嗜酸菌，最适生长pH为3.0。它可能通过在培养过程中产酸来调节pH值。在pH 3.0条件下，菌株ZM 1对1100 mg/L苯酚的降解率最高，为0.074 g/（g菌体干重·h），在120 h内几乎完全降解。在相同pH条件下，当NaCl浓度高达10 g/L时，该菌株可在48 h内完全降解500 mg/L的苯酚。通过KEGG分析对genecatA的鉴定，以及气相色谱-质谱联用检测到的代谢中间产物cis，cis-粘康酸的存在，证实菌株ZM 1通过邻位裂解途径降解苯酚。这些结果表明，土著酵母菌株ZM 1可以被开发作为一个重要的成员，原位降解芳香族化合物在极端酸性环境中。
The goal of this work was to investigate the genetics of an acidophilic phenol-degrading yeast strain using whole-genome sequencing (WGS), characterize the growth of the strain and phenol degradation capability as well as degradation pathway under extremely acidic conditions. The result showed that the strain ZM1 isolated from an acid mine drainage (AMD) belongs to basidiomycetous yeastRhodotorulasp., which possesses some unique genes compared to other four closely relatedRhodotorulaspecies. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that ZM1 possessed the degradation potentials for aromatic compounds. ZM1 was acidophilic with the optimum growth at the initial pH of 3.0. It could adjust pH to desired levels probably by acid production during the cultivation. Notably, at pH 3.0, the strain ZM1 showed a high phenol-degrading capability that almost completely degraded 1100 mg/L of phenol in 120 h with the highest degradation rate of 0.074 g/(g cell dry weight h). Under the same pH, the strain could completely degrade 500 mg/L phenol within 48 h at NaCl concentration up to 10 g/L. The identification of the genecatAby the KEGG analysis, together with the presence of metabolic intermediate ofcis, cis-muconic acid detected by gas chromatography-mass spectrometry, confirmed that the strain ZM1 degraded phenol viaortho-cleavage pathway. These findings suggest that the indigenous yeasts strain ZM1 could be exploited as an important member for in-situ biodegradation of aromatic compounds in the extremely acidic environments.