T cell recognition of Mlsc. I. Influence of MHC gene products in Mlsc-specific T cell recognition.
T cell recognition of Mlsc. I. Influence of MHC gene products in Mlsc-specific T cell recognition.
复制标题
Mlsc 的 T 细胞识别。
作者：
R. Abe;R. Hodes
单特异性T细胞克隆已被证明是在许多Ag特异性以及同种异体特异性T细胞应答中表征T细胞识别的有力工具。为了阐明T细胞识别次要刺激位点Ag（Mlsc）决定簇的机制，本研究采用Mlsc特异性克隆T细胞和原代T细胞应答，以阐明MHC基因产物在Mlsc特异性T细胞识别中的作用。结果表明，T细胞识别Mlsc决定簇结合I区MHC基因产物。此外，某些MHC单倍型（例如，H-2a和H-2k）似乎在Mlsc的“呈递”中有效地起作用，而其他单倍型（例如，H-2b和H-2 q）在呈现Mlsc中的功能很差（如果有的话）。使用来源于低刺激性H-2b，Mlsc菌株C3H.SW和一组Mlsb，H-2-不同或H-2内重组菌株之间的F1杂交体的刺激物的实验强烈地表明，刺激物上E α E β分子的表达对Mlsc刺激起关键作用。通过抗-E α单克隆抗体抑制克隆的Mlsc特异性T细胞的应答的能力进一步证明了E α E β产物在Mlsc识别中的功能重要性。抗A β k抗体对相同的Mlsc特异性应答的抑制表明A β产物也可能在T细胞对Mlsc的应答中起作用。
Monospecific T cell clones have been proven to be powerful tools for the characterization of T cell recognition in many Ag-specific as well as allo-specific T cell responses. In this report, in order to elucidate the mechanism of T cell recognition of minor stimulating locus Ag (Mlsc) determinants, Mlsc-specific cloned T cells were employed together with primary T cell responses to clarify the role of MHC-gene products in Mlsc-specific T cell recognition. The results indicated that T cells recognize Mlsc determinants in conjunction with I-region MHC gene products. Moreover, certain MHC haplotypes (e.g., H-2a and H-2k) appear to function efficiently in the "presentation" of Mlsc, whereas other haplotypes (e.g., H-2b and H-2q) function poorly if at all in presenting Mlsc. Experiments with the use of stimulators derived from F1 hybrids between the low stimulatory H-2b, Mlsc strain, C3H.SW, and a panel of Mlsb, H-2-different or intra-H-2 recombinant strains strongly suggested that expression of E alpha E beta molecules on stimulators plays a critical role for Mlsc stimulation. The functional importance of the E alpha E beta product in Mlsc recognition was further demonstrated by the ability of anti-E alpha monoclonal antibody to inhibit the response of cloned Mlsc-specific T cells. Inhibition of the same Mlsc-specific response by anti-A beta k antibody suggests that the A beta product may also play a role in T cell responses to Mlsc.