GROWTH OF UROPATHOGENIC ESCHERICHIA-COLI STRAINS AT SOLID-SURFACES
GROWTH OF UROPATHOGENIC ESCHERICHIA-COLI STRAINS AT SOLID-SURFACES
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DOI：
10.1163/156856292x00213
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发表时间：
1992-01-01
影响因子：
3.6
通讯作者：
FEIJEN, J
中科院分区：
文献类型：
--
作者：
HARKES, G;DANKERT, J;FEIJEN, J
两种导管相关的粘附和生长（O2 K2和O 83 K？）研究了两株非导管相关性（O 111 K58和O 157 K-）尿路致病性大肠杆菌菌株在玻璃、聚甲基丙烯酸甲酯（PMMA）、带负电荷的MMA和甲基丙烯酸共聚物（MAA）以及带正电荷的MMA和甲基丙烯酸三甲基氨基乙酯氯化物共聚物（TMAEMA-Cl）上的粘附。 将固体表面置于平行板灌注系统中。在细菌预粘附到表面上之后，通过用麦康凯肉汤灌注系统来启动生长。通过计数粘附细菌作为时间的函数来测量生长。通过水接触角、微生物对烃的粘附性（MATH）、阴离子交换树脂保留率（ARR）和zeta电位测量对细菌菌株进行了表征。固体表面，其特征在于通过水接触角和zeta电位测量。 导管相关菌株的水接触角、zeta电位和ARR值显著高于非导管相关菌株。非导管相关菌株未在所用表面生长。导管相关菌株没有在带正电的表面生长，但在其他表面生长。菌株生长更迅速地在一个相对较高的负zeta电位和一个低的水接触角的表面比在一个相对较低的负zeta电位和一个高的水接触角的表面。当以尿液代替麦康凯肉汤作为灌注培养基时，菌株O2 K2在玻璃上的生长显著减少。
The adhesion and growth of two catheter-associated (O2K2 and O83K?) and two non catheter-associated (O111K58 and O157K-) uropathogenic Escherichia coli strains on glass, poly(methyl methacrylate) (PMMA), a negatively charged copolymer of MMA and methacrylic acid (MAA) and a positively charged copolymer of MMA and trimethylaminoethyl methacrylate chloride (TMAEMA-Cl) were studied. The solid surfaces were placed in a parallel plate perfusion system. After preadhesion of the bacteria onto the surfaces, growth was initiated by perfusing the system with MacConkey broth. Growth was measured by counting adherent bacteria as a function of time. Bacterial strains were characterized by means of water contact angle, microbial adhesion to hydrocarbon (MATH), anion exchange resin retention (ARR) and zeta potential measurements. Solid surfaces were characterized by means of water contact angle and zeta potential measurements. The catheter-associated strains had significantly higher water contact angles, zeta potentials and ARR values than the non catheter-associated strains. Non catheter-associated strains did not grow at the surfaces used. Catheter-associated strains did not grow at the positively charged surface but exhibited growth at the other surfaces. Strains grew more rapidly at surfaces with a relatively high negative zeta potential and a low water contact angle than at surfaces with a relatively low negative zeta potential and a high water contact angle. The growth of strain O2K2 on glass was significantly reduced when urine instead of MacConkey broth was used as perfusion medium.