Bioimaging TOF-SIMS: High resolution 3D Imaging of single cells
Bioimaging TOF-SIMS: High resolution 3D Imaging of single cells
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DOI：
10.1002/jemt.20502
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发表时间：
2007-11-01
影响因子：
2.5
通讯作者：
Richter, Katrin
中科院分区：
文献类型：
--
作者：
Nygren, Hakan;Hagenhoff, Birgit;Richter, Katrin
利用成像飞行时间二次离子质谱（TOF - SIMS）分析了甲状腺肿瘤细胞中磷酸胆碱离子（质荷比184、质荷比86）、钠离子和钾离子的分布。用C - 60（+）源重复溅射，随后用Bi - 3（+）枪进行分析，产生了一系列138张图像，这些图像堆叠后形成细胞化学的三维显示。在质膜（质荷比184）和细胞内膜（质荷比86）中观察到磷酸胆碱。磷脂的不同裂解可能反映了这些部位膜的化学成分。在有膜包围的细胞区室中观察到高强度的钾二次离子信号。数据表明钾离子在甲状腺肿瘤细胞中是区室化分布的。
The distribution of phosphocholine ions (m/z 184, m/z 86), sodium ions, and potassium ions in thyroid tumor cells was analyzed by imaging TOF-SIMS. Repeated sputtering with a C-60(+) source and subsequent analysis with a Bi-3(+) gun produced a series of 138 images that were stacked to make a 3D display of the chemistry of cells. Phosphocholine was seen in the plasma membrane (m/z 184) and intracellular membranes (m/z 86). The different fragmentation of the phospholipid probably reflects the chemical composition of membranes at these sites. High intensity of secondary ion signals of potassium was seen in membrane-encompassed cellular compartments. The data indicate that potassium ions are compartmentalized in thyroid tumor cells.