Impact of wet-dry cycling on the phase behavior and compartmentalization properties of complex coacervates.
Impact of wet-dry cycling on the phase behavior and compartmentalization properties of complex coacervates.
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DOI：
10.1038/s41467-020-19184-z
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发表时间：
2020-10-27
影响因子：
16.6
通讯作者：
Keating CD
中科院分区：
文献类型：
--
作者：
Fares HM;Marras AE;Ting JM;Tirrell MV;Keating CD
早期地球上的干湿循环被认为促进了生命分子构建模块的产生，但其对自组装和划分的影响在很大程度上仍未被探索。在这里，我们研究脱水/复水的复凝聚层，这是无膜隔室形成的相分离的水溶液。鉴定了十倍失水导致凝聚液滴维持、消失或出现的溶液组合物。系统保持凝聚体在整个脱水过程中进一步评估，以了解其分区的性质如何改变干燥。虽然加入的总RNA浓度增加了10倍，但凝聚层内的RNA浓度保持稳定。外部RNA浓度上升，和交换率封装与游离RNA增加脱水。我们解释这些结果的相图，与脱水驱动的离子强度增加是特别重要的，在确定凝聚性能。这项工作表明，干湿循环可以改变相行为和复合凝聚层的原细胞相关功能。干湿循环被认为能够产生生命的分子构建模块。在这里，作者研究了脱水/再水化对含RNA的复合凝聚体的影响，复合凝聚体是由水溶液的相分离形成的无膜隔室。
Wet-dry cycling on the early Earth is thought to have facilitated production of molecular building blocks of life, but its impact on self-assembly and compartmentalization remains largely unexplored. Here, we investigate dehydration/rehydration of complex coacervates, which are membraneless compartments formed by phase separation of polyelectrolyte solutions. Solution compositions are identified for which tenfold water loss results in maintenance, disappearance, or appearance of coacervate droplets. Systems maintaining coacervates throughout the dehydration process are further evaluated to understand how their compartmentalization properties change with drying. Although added total RNA concentrations increase tenfold, RNA concentration within coacervates remains steady. Exterior RNA concentrations rise, and exchange rates for encapsulated versus free RNAs increase with dehydration. We explain these results in light of the phase diagram, with dehydration-driven ionic strength increase being particularly important in determining coacervate properties. This work shows that wet-dry cycling can alter the phase behavior and protocell-relevant functions of complex coacervates. Wet-dry cycling is thought to have enabled the production of molecular building blocks of life. Here, the authors investigate the impact of dehydration/rehydration on RNA-containing complex coacervates, which are membraneless compartments formed by phase separation of polyelectrolyte solutions.
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影响因子：
16.6
作者：
Campbell, Thomas D.;Febrian, Rio;Bracher, Paul J.
通讯作者：
Bracher, Paul J.
影响因子：
1.7
作者：
Fox, Stefan;Pleyer, Hannes Lukas;Strasdeit, Henry
通讯作者：
Strasdeit, Henry
影响因子：
21.8
作者：
Aumiller, William M., Jr.;Keating, Christine D.
通讯作者：
Keating, Christine D.
影响因子：
16.6
作者：
Drobot B;Iglesias-Artola JM;Le Vay K;Mayr V;Kar M;Kreysing M;Mutschler H;Tang TD
通讯作者：
Tang TD
影响因子：
16.6
作者：
Chang LW;Lytle TK;Radhakrishna M;Madinya JJ;Vélez J;Sing CE;Perry SL
通讯作者：
Perry SL