Functional magnetic resonance imaging in rodents: Methodology and application to spinal cord injury
Functional magnetic resonance imaging in rodents: Methodology and application to spinal cord injury
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DOI：
10.1002/jnr.21030
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发表时间：
2006-11-01
影响因子：
4.2
通讯作者：
Narayana, Ponnada A.
中科院分区：
文献类型：
--
作者：
Ramu, Jaivijay;Bockhorst, Kurt H.;Narayana, Ponnada A.
对脊髓损伤后4周和8周的功能磁共振成像(FMRI)进行了研究。基于对大鼠爪子的电刺激，fMRI的范例是使用内部设计的基于微处理器的控制器与刺激器接口实现自动化的。使用公开可用的冷冻切片的数字图像，将MR图像空间归一化到Paxinos和Watson图谱。在正常的未受伤动物中，激活仅限于对侧的躯体感觉皮质。相反，在受伤的动物中，在第4周和第8周观察到广泛的激活，包括同侧皮质、丘脑、海马体和尾壳核等结构。定量聚类分析用于计算单个脑结构的激活体积和激活中心。基于这一分析，在4至8周期间，仅观察到同侧尾壳核和丘脑的激活显著增加。这些研究表明，脊髓损伤动物的大脑进行了广泛和持续的重组。(C)2006年Wiley-Liss，Inc.
Functional MRI (fMRI) on spinal cord-injured rodents at 4 and 8 weeks post injury (PI) is described. The paradigm for fMRI, based on electrical stimulation of rat paws, was automated using an in-house designed microprocessor-based controller that was interfaced to a stimulator. The MR images were spatially normalized to the Paxinos and Watson atlas using publicly available digital images of the cryosections. In normal uninjured animals, the activation was confined to the contralateral somatosensory cortex. In contrast, in injured animals, extensive activation, which included structures such as ipsilateral cortex, thalamus, hippocampus, and the caudate putamen, was observed at 4 and 8 weeks Pl. Quantitative cluster analysis was carried out to calculate the volumes and centers of activation in individual brain structures. Based on this analysis, significant increase in activation between 4 and 8 weeks was observed only in the ipsilateral caudate putamen and thalamus. These studies suggest extensive and ongoing brain reorganization in spinal cord-injured animals. (c) 2006 Wiley-Liss, Inc.