THE INFLUENCE OF SUBSTRATUM SURFACE FREE-ENERGY ON GROWTH AND SPREADING OF HUMAN-FIBROBLASTS IN THE PRESENCE AND ABSENCE OF SERUM-PROTEINS
THE INFLUENCE OF SUBSTRATUM SURFACE FREE-ENERGY ON GROWTH AND SPREADING OF HUMAN-FIBROBLASTS IN THE PRESENCE AND ABSENCE OF SERUM-PROTEINS
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DOI：
10.1002/jbm.820200609
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发表时间：
1986-07-01
期刊：
影响因子：
--
通讯作者：
ARENDS, J
中科院分区：
文献类型：
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作者：
SCHAKENRAAD, JM;BUSSCHER, HJ;ARENDS, J
为了确定高分子材料的表面自由能是否影响细胞的铺展和生长，13种聚合物和玻璃的表面自由能与人皮肤成纤维细胞的铺展和生长有关。在存在和不存在血清蛋白的情况下进行实验。我们从磷酸盐缓冲盐水（PBS）、正丙醇/PBS混合物和α-聚乙二醇的接触角计算表面自由能。溴萘对聚合物的影响，使用极性和分散组分的概念解释扩散压力。细胞铺展和基质表面自由能（γ s）在存在和不存在血清蛋白的情况下均显示出特征性的S形关系;仅当γ s高于约57 erg·cm·t时才发生良好铺展。厘米-2。在存在血清蛋白的情况下，细胞铺展在大多数材料上是相似的，只有少数材料显示出相对较高的细胞铺展。在血清蛋白存在下的细胞生长在各种聚合物上就其γ s值而言没有显著差异。相比之下，两组聚合物可以区分在血清的情况下，相对于细胞生长。第一组显示出随着γ s的增加而增加的细胞生长，而第二组显示出一致的低细胞生长。结果表明，细胞的传播和基质的表面自由能之间的复杂关系，以及血清蛋白质的作用，在修改的表面特性的聚合物在细胞的传播和生长。
To determine whether the surface free energy of polymer materials influences the spreading and growth of cells, surface free energies of 13 polymers and glass were related to spreading and growth of human skin fibroblasts. Experiments were performed in both the presence and absence of serum proteins. We calculated the surface free energy from contact angles of phosphate-buffered saline (PBS), n-propanol/PBS mixtures, and .alpha.-bromonaphthalene on the polymers, using the concept of polar and dispersion components accounting for spreading pressures. Cell spreading and substratum surface free energy (.gamma.s) showed a characteristic sigmoid relationship both in the presence and in the absence of serum proteins; good spreading only occurred when .gamma.s was higher than approximately 57 erg .cntdot. cm-2. In the presence of serum proteins, cell spreading is similar on most materials, only few materials show relatively high cell spreading. Cell growth in the presence of serum proteins did not differ significantly on the various polymers with reference to their .gamma.s values. In contrast, two groups of polymers could be distinguished in the absence of serum with respect to cell growth. The first group showed increasing cell growth with increasing .gamma.s, whereas the second group showed consistently low cell growth. The results demonstrate the complex relationship between cell spreading and substratum surface free energy as well as the role of serum proteins in modifying the surface characteristics of polymers in relation to cell spreading and growth.