Notch pathway repression by vestigial is required to promote indirect flight muscle differentiation in Drosophila melanogaster
Notch pathway repression by vestigial is required to promote indirect flight muscle differentiation in Drosophila melanogaster
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DOI：
10.1016/j.ydbio.2006.03.022
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发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Lalouette, A.
中科院分区：
文献类型：
--
作者：
Bernard, F.;Dutriaux, A.;Lalouette, A.
果蝇背纵肌在变态过程中通过成肌细胞与幼虫模板融合而发育。研究表明，残余肌和切迹肌对这些肌肉的正确形成至关重要。我们研究了在这个过程中，退化和Notch通路之间的关系。使用分裂区转录m6基因表达增强子作为Notch途径活性的报告基因，我们能够证明该途径仅在成肌细胞中有活性。此外，在背纵肌发育过程中，对N通路的几个主要参与者（Notch、Delta、neuralized、Serrate、Mind bomb 1和fringe）的细胞位置的仔细检查使我们能够发现Notch受体在成人肌发生中可以发挥多种作用。我们报告，两个Notch配体（Delta和Serrate）的位置是不同的。有趣的是，我们发现，编码糖基转移酶的条纹，改变了Notch受体对其配体的亲和力，在肌纤维和成肌细胞的子集中表达。此外，我们证明了边缘表达对于Notch途径抑制和肌肉分化是必不可少的。最后，我们报告说，在退化的突变体，条纹表达丢失，当条纹过表达，一个显着的救援间接飞行肌变性。总之，我们的数据表明，通过抑制Notch途径实现了残留分化功能。(c)2006年爱思唯尔公司All rights reserved.
Drosophila dorsal longitudinal muscles develop during metamorphosis by fusion of myoblasts with larval templates. It has been shown that both vestigial and Notch are crucial for correct formation of these muscles. We investigated the relationship between vestigial and the Notch pathway during this process. Using Enhancer of Split Region Transcript m6 gene expression as a reporter of Notch pathway activity, we were able to demonstrate that this pathway is only active in myoblasts. Moreover, close examination of the cellular location of several of the main actors of the N pathway (Notch, Delta, neuralized, Serrate, Mind bomb1 and fringe) during dorsal longitudinal muscle development enabled us to find that Notch receptor can play multiple roles in adult myogenesis. We report that the locations of the two Notch ligands (Delta and Serrate) are different. Interestingly, we found that fringe, which encodes a glycosyltransferase that modifies the affinity of the Notch receptor for its ligands, is expressed in muscle fibers and in a subset of myoblasts. In addition, we demonstrate that fringe expression is essential for Notch pathway inhibition and muscle differentiation. Lastly, we report that, in vestigial mutants, fringe expression is lost, and when fringe is overexpressed, a significant rescue of indirect flight muscle degeneration is obtained. Altogether, our data show that a vestigial-differentiating function is achieved through the inhibition of the Notch pathway. (c) 2006 Elsevier Inc. All rights reserved.