Polyamines as protectors against cadmium or copper-induced oxidative damage in sunflower leaf discs
Polyamines as protectors against cadmium or copper-induced oxidative damage in sunflower leaf discs
复制标题
DOI：
10.1016/s0168-9452(01)00432-0
复制
发表时间：
2001-08-01
期刊：
影响因子：
5.2
通讯作者：
Benavides, MP
中科院分区：
文献类型：
--
作者：
Groppa, MD;Tomaro, ML;Benavides, MP
研究了镉和铜诱导的氧化胁迫下向日葵叶盘中多胺的抗氧化特性。两种金属均可引起组织的氧化损伤，表现为脂质过氧化作用增强，抗氧化酶抗坏血酸过氧化物酶(APOX)、谷胱甘肽还原酶(GR)和超氧化物歧化酶(SOD)活性下降。多胺浓度根据所用金属的不同而受到不同的影响。Cd~(2+)使腐胺(Put)降低52%，Cu2+使腐胺(Put)降低39%，Cd~(2+)和Cu~(2+)分别使精胺(Spd)降低到对照的21%和59%，精胺(Spm)含量不受任何金属的影响。加入外源多胺(1 MM)后，SPM可使Cd~(2+)、Cu~(2+)对脂质过氧化的影响几乎恢复到对照水平。SPM和SPD均不能恢复金属降低的APOX活性。此外，两种多胺都单独影响这一参数。SPM或SPD均能完全恢复GR活性，而只有SPM能有效逆转铜降低的SOD活性。这项工作表明，多胺无疑与防止金属诱导的氧化应激有关，但需要更多的研究来阐明它们作为抗氧化剂所起的确切作用。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
Polyamines antioxidant properties were studied in sunflower leaf discs under cadmium and copper induced oxidative stress. Both metals induced oxidative damage in the studied tissue, as evidenced by an increment in lipid peroxidation and a decrease in antioxidant enzymes ascorbate peroxidase (APOX), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Polyamines concentrations were differently affected according to the metal used. Putrescine (Put) was reduced 52% by Cd2+ and 39% by Cu2+, spermidine (Spd) was decreased to 21 and 59% of the control by Cd2+ and Cu2+, respectively and spermine (Spm) content was not modified by any of the metals. Pretreatment with exogenously added polyamines (1 mM) showed that Spm reverted the effect of Cd2+ and Cu2+ on lipid peroxidation almost to control values. Neither Spm nor Spd recovered the metal-reduced APOX activity. Besides, both polyamines affected this parameter by themselves. GR activity was completely restored by Spm or Spd and only Spm was effective in reverting copper-reduced SOD activity. This work suggests that polyamines are undoubtedly related to the protection against metal-induced oxidative stress, but more research is necessary to elucidate the precise role they played as antioxidants. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.