Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture
Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture
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DOI：
10.1089/ten.tea.2019.0188
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发表时间：
2019-12-27
影响因子：
4.1
通讯作者：
Steinke, Maria
中科院分区：
文献类型：
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作者：
Lodes, Nina;Seidensticker, Katharina;Steinke, Maria
已经使用例如人原代气道上皮细胞（hAEC）或相应的细胞系生成了三维呼吸组织模型。研究纤毛病，如原发性纤毛运动障碍，功能性动纤毛在体外的存在是一个必要的先决条件。由于获得健康供体的hAEC是有限的，我们的目的是确定一个呼吸道上皮细胞系，能够显示至少60%的顶端表面上的功能动纤毛。因此，我们培养四种不同的人呼吸道细胞系与人原代气道成纤维细胞在气升条件下，其特征在于形态，并分析纤毛功能。只有一个测试的细胞系显示跳动的动纤毛;然而，
Three-dimensional respiratory tissue models have been generated using, for example, human primary airway epithelial cells (hAEC) or respective cell lines. To investigate ciliopathies, such as primary ciliary dyskinesia, the presence of functional kinocilia in vitro is an essential prerequisite. Since access to hAEC of healthy donors is limited, we aimed to identify a respiratory epithelial cell line that is capable to display functional kinocilia on at least 60% of the apical surface. Thus, we cultured four different human respiratory cell lines with human primary airway fibroblasts under airlift conditions, characterized the morphology, and analyzed ciliary function. Only one of the tested cell lines showed beating kinocilia; however,