MANIPULATION OF INTRACELLULAR MAGNESIUM CONTENT IN POLYMYXIN-B NONAPEPTIDE-SENSITIZED ESCHERICHIA-COLI BY IONOPHORE A23187
MANIPULATION OF INTRACELLULAR MAGNESIUM CONTENT IN POLYMYXIN-B NONAPEPTIDE-SENSITIZED ESCHERICHIA-COLI BY IONOPHORE A23187
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DOI：
10.1128/jb.162.1.413-419.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
KELLENBERGER, E
中科院分区：
文献类型：
--
作者：
ALATOSSAVA, T;JUTTE, H;KELLENBERGER, E
用多粘菌素B九肽敏化大肠杆菌B细胞对离子载体A23187的敏感性，并研究其对镁离子和钾离子的诱导通量。联合离子载体处理以离子特异性的方式使大肠杆菌的细胞膜通透性，并允许从外部操纵细胞内的镁含量。A23187诱导的镁离子外流或内流依赖于细胞膜内外的游离镁离子浓度梯度和pH梯度。胞液中大部分镁离子是结合在一起的，而胞液中只有1-2 mM的游离镁离子。
E. coli B cells were sensitized to ionophore A23187 by polymyxin B nonapeptide, and the induced Mg and K ion fluxes were studied. Combined ionophore treatment permeabilized the cytoplasmic membrane of E. coli in an ion-specific manner and allowed the manipulation of intracellular Mg2+ content from the outside. A23187-induced Mg2+ efflux or influx was dependent on the free Mg2+ concentration gradient between the outside and inside of the cytoplasmic membrane and on the pH gradient. Most of the intracellular Mg2+ was bound, whereas only 1-2 mM was free in solution in the cellular sap.