Site-specific development of periodontal disease is associated with increased levels of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque
Site-specific development of periodontal disease is associated with increased levels of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque
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DOI：
10.1902/jop.2008.070398
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发表时间：
2008-04-01
影响因子：
4.3
通讯作者：
Takehara, Tadarnichi
中科院分区：
文献类型：
--
作者：
Mineoka, Tetsuro;Awano, Shuji;Takehara, Tadarnichi
背景：牙龈卟啉单胞菌、齿密螺旋体和连翘坦纳氏菌被认为是主要的牙周病原菌，它们以联合体的形式存在于龈下生物膜中。我们的目的是检测牙龈下生物膜中牙龈假单胞菌、齿状念珠菌和连翘之间的定量关系，以及这三种细菌的数量和流行率与特定部位的牙周健康的关系。研究人群包括35名访问九州牙科大学医院的成年受试者。从105个牙周袋部位采集菌斑样本。结果：菌斑样本根据有无牙周袋(探诊深度=4 mm)和探诊时出血(BOP)分为4组，与附着丧失无关。在牙周病区(PD和BOP)标本中检出的三种靶菌比例均明显高于其他样本组。牙周病各采样点菌斑中牙龈假单胞菌、齿状念珠菌和连翘的菌落数呈显著正相关，且在牙周病患区菌斑中呈增加趋势。结论：牙龈假单胞菌、齿假单胞菌和连翘共生作用可能与局部牙周炎的发生发展有关。
Background: Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (previously T. forsythensis), which are regarded as the principal periodontopathogenic bacteria, exist as a consortium in subgingival biofilms. We aimed to examine quantitative relationships between P. gingivalis, T. denticola, and T. forsythia in subgingival biofilms and the relationship between the quantity and prevalence of these three bacteria and site-specific periodontal health.Methods: This study was cross - sectional. The study population consisted of 35 adult subjects who visited the Kyushu Dental College Hospital. Plaque samples were collected from 105 periodontal pocket sites. Quantitative analyses of each of the three periodontopathogenic bacteria were performed using real-time polymerase chain reaction with species-specific primers and hybridization probes.Results: The plaque samples were divided into four groups based on the presence or absence of a periodontal pocket (probing depth [PD] >= 4 mm) and bleeding on probing (BOP), regardless of attachment loss. The proportions of all three target bacteria detected in samples from sites of periodontal disease (with PD and BOP) were markedly higher than those in the other sample groups. Cell numbers of P. gingivalis, T. denticola, and T. forsythia in the subgingival plaque of each sampling site were significantly mutually correlated and were increased in the plaque of sites of periodontal disease with PD >= 4 mm and BOP.Conclusion: The symbiotic effects of P. gingivalis, T. denticola, and T. forsythia, which coaggregate and exist concomitantly in subgingival biofilms, may be associated with the local development of periodontitis.