Persistence and spatial variation of antibiotic resistance genes and bacterial populations change in reared shrimp in South China
Persistence and spatial variation of antibiotic resistance genes and bacterial populations change in reared shrimp in South China
复制标题
华南养殖对虾抗生素抗性基因的持久性和空间变异及细菌种群变化
DOI：
10.1016/j.envint.2018.07.007
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发表时间：
2018
影响因子：
11.8
通讯作者：
Yucheng Cao
中科院分区：
文献类型：
--
作者：
Haochang Su;Xiaojuan Hu;Yu Xu;Wujie Xu;Xiaoshuai Huang;Guoliang Wen;Keng Yang;Zhuojia Li;Yucheng Cao
水生环境中抗生素耐药基因（ARGs）的丰度和多样性越来越受到人们的关注。然而，关于ARGs在水生生物体内的持久性和空间变异的研究很少。本研究调查了广东不同地区对虾养殖期间肠道中ARGs的发生、丰度和细菌种群数量。结果表明，sul1、sul2、qnrD和flor是主要的ARGs。与幼虾相比，3个养殖场的成年对虾肠道ARGs总浓度高2.45 ~ 3.92倍（p< 0.05），且成年对虾肠道菌群变化较大。冗余分析（RDA）显示，A、B和C养殖场中Proteobacteria、Firmicutes和Verrucomicrobia的丰度与A养殖场中最丰富和最优势的基因（sul1和qnrd， floR和sul2， floR和sul2， C养殖场）呈显著正相关。本研究结果表明，在养殖对虾的发育阶段，ARGs具有持久性。不同的优势菌门对不同地区对虾肠道中ARGs丰度的增加有不同的作用。本研究为水产养殖中ARGs的持久性和空间变异提供了案例研究，可为确定ARGs对食品安全和人体健康的有害影响提供参考。
More attention has been paid to the abundance and diversity of antibiotic resistance genes (ARGs) in aquatic environments. However, few studies have investigated the persistence and spatial variation of ARGs in aquatic organisms. This study investigated the occurrence and abundance of ARGs and the bacterial populations in shrimp intestinal tracts during the rearing period in different regions of Guangdong, South China. The results showed thatsul1,sul2,qnrD, andfloR were the predominant ARGs. Compared with those of juvenile shrimp, the total concentrations of ARGs in the intestinal tract of adult shrimp in three shrimp farms were 2.45–3.92 times higher (p< 0.05), and the bacterial populations in the adult shrimp intestinal tract changed considerably. Redundancy analysis (RDA) showed that the abundance of Proteobacteria, Firmicutes, and Verrucomicrobia in Farms A, B, and C, respectively, were strongly positively correlated with the most abundant and predominant genes (sul1 andqnrD for Farm A;floR andsul2 for Farm B;floR andsul2 for Farm C) in the shrimp intestinal tract. The results of this study indicated that ARGs gained persistence in the developmental stages of the reared shrimp. Different phyla of predominant bacteria were responsible for the increase of ARGs abundance in the shrimp intestinal tract in different regions. This study represents a case study of the persistence and spatial variation of ARGs in aquaculture and can be a reference for the determination of harmful impacts of ARGs on food safety and human health.