Wilms' tumour 1 gene expression is increased in hepatocellular carcinoma and associated with poor prognosis
Wilms' tumour 1 gene expression is increased in hepatocellular carcinoma and associated with poor prognosis
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DOI：
10.1016/j.ejca.2008.01.008
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发表时间：
2008-03-01
影响因子：
8.4
通讯作者：
Onji, Morikazu
中科院分区：
文献类型：
--
作者：
Sera, Toshiki;Hiasa, Yoichi;Onji, Morikazu
Background/aims: Wilms' tumour 1 gene(WT1) was originally isolated as a tumour-suppressor gene. We investigated the expression of WT1 in hepatocellular carcinoma (HCC; T) and in non-cancerous hepatic tissues (non-tumour: NT) from patients with chronic liver diseases, and then examined the role of WT1 in the carcinogenesis or prognosis of HCC.Methods: The expression of WT1 in T and NT from 50 patients with HCC was investigated using Western blotting, immunohistochernistry and real-time reverse transcriptase-polymerase chain reaction (RT-PCR). We also examined whether WT1 expression was related to clinicopathological factors in individual patients in addition to prognostic factors in 50 patients with HCC and in 26 without HCC.Results: Western blotting and immunohistochemical staining showed that WT1 was over-expressed in T compared with NT (P < 0.001) and real-time RT-PCR showed that WT1 mRNA expression was similarly increased. Overexpressed WT1 in HCC was significantly associated with T factors at the TNM stage, and short doubling time of HCC. Univariate and multivariate analyses revealed that WT1 overexpression. was an independent prognostic factor for HCC. The disease-free survival period in patients with overexpressed WT1 in NT tissues was significantly reduced.Conclusion: The expression of WT1 is increased more in HCC than in non-tumour tissues. Moreover, overexpressed WT1 was associated with tumour growth, and resulted in a worsening prognosis of HCC. Our findings from NT tissues revealed that WT1 overexpression might contribute to oncogenic potential. (c) 2008 Elsevier Ltd. All rights reserved.
