Visualization and Semiquantitative Study of the Distribution of Major Components in Wheat Straw in Mesoscopic Scale using Fourier Transform Infrared Microspectroscopic Imaging
Visualization and Semiquantitative Study of the Distribution of Major Components in Wheat Straw in Mesoscopic Scale using Fourier Transform Infrared Microspectroscopic Imaging
复制标题
利用傅里叶变换红外显微光谱成像对小麦秸秆中主要成分在细观尺度上的分布进行可视化和半定量研究
DOI：
10.1021/acs.analchem.8b00614
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发表时间：
2018
影响因子：
7.4
通讯作者：
Han Lujia
中科院分区：
文献类型：
--
作者：
Yang Zengling;Mei Jiaqi;Liu Zhiqiang;Huang Guangqun;Huang Guan;Han Lujia
了解与作物秸秆解剖相关的植物组织的生化异质性对植物研究人员和生物质精炼领域的研究人员具有吸引力。采用快速非负约束最小二乘法(FAST NNLS)对基于傅里叶变换红外(FTIR)显微光谱成像的麦草节间横切面主要成分分布进行了原位分析和半定量可视化。本文研究了小麦拔节期、孕穗期、抽穗期、开花期、灌浆期、乳熟期、面团成熟期和成熟期组织生化成分的变化。对麦草的5种成分(微晶纤维素、木聚糖、木质素、果胶和淀粉)进行了参考光谱的可视化分析。结果表明：(A)纤维素和木质素的分布与藏红花O-固绿组织染色的分布一致；(B)纤维素、木质素和淀粉的分布分别在1432、1507和987 cm-1的特征波长上与化学图像一致，表明所分析的其他组分没有干扰。在利用特征波长和组织染色技术对生化图像进行验证的基础上，进一步基于FAST NNLS对局部组织进行了半定量分析，结果表明：(A)不同组织中的纤维素含量差异很大，以薄壁组织最多，表皮最少；(B)在植物发育过程中，组织中各组分的变化趋势基本一致，尤其是维管束和薄壁组织。因此，FTIR显微光谱成像结合合适的化学计量学方法可以成功地研究小麦秸秆节间横截面内的化学分布，提供半定量的化学信息。
Understanding the biochemical heterogeneity of plant tissue linked to crop straw anatomy is attractive to plant researchers and researchers in the field of biomass refinery. This study provides an in situ analysis and semiquantitative visualization of major components distribution in internodal transverse sections of wheat straw based on Fourier transform infrared (FTIR) microspectroscopic imaging, with a fast non-negativity-constrained least squares (fast NNLS) fitting. This paper investigates changes in biochemical components of tissue during stages of elongation, booting, heading, flowering, grain-filling, milk-ripening, dough, and full-ripening. Visualization analysis was carried out with reference spectra for five components (microcrystalline cellulose, xylan, lignin, pectin, and starch) of wheat straw. Our result showed that (a) the cellulose and lignin distribution is consistent with that from tissue-dyeing with safranin O-fast green and (b) the distribution of cellulose, lignin, and starch is consistent with chemical images for characteristic wavelength at 1432, 1507, and 987 cm–1, respectively, showing no interference from the other components analyzed. With the validation from biochemical images using characteristic wavelength and tissue-dyeing techniques, further semiquantitative analysis in local tissues based on fast NNLS was carried out, and the result showed that (a) the contents of cellulose in various tissues are very different, with most in parenchyma tissue and least in the epidermis and (b) during plant development, the fluctuation of each component in tissues follows nearly the same trend, especially within vascular bundles and parenchyma tissue. Thus, FTIR microspectroscopic imaging combined with suitable chemometric methods can be successfully applied to study chemical distributions within the internodes transverse sections of wheat straw, providing semiquantitative chemical information.