Molecular interferometric imaging.
Molecular interferometric imaging.
复制标题
分子干涉成像。
作者：
Ming Zhao;Xuefeng Wang;D. Nolte
共路径在线剪切干涉测量与像素阵列成像相结合，提供了可实现 15 pm 表面高度分辨率的表面计量。硅上的第八波热氧化物生成锁定在相位正交条件下的参考波，以进行相位到强度的转换，从而使成像系统可以直接检测到表面高度或折射率变化。表面计量应用的缩放表面质量灵敏度在 40 倍放大倍数下为 S(scal) = 7 fg/mm，像素内的分子分辨率约为 12 个 IgG 分子，但受基材表面粗糙度的限制。当在 7 倍放大倍数下应用于抗体微阵列形式的反相免疫测定时，受生物和化学变异性的限制，1 小时孵育的当前检测限为 10 ng/ml。该生物传感器与主动流条件下的实时结合测量兼容，每孔的结合动态范围为 10(3)，质量灵敏度为 2 pg/mm(2)。
Common-path in-line shearing interferometry, combined with pixel-array imaging, provides a surface metrology that achieves 15 pm surface height resolution. An eighth-wave thermal oxide on silicon generates a reference wave locked in the condition of phase quadrature for phase-to-intensity conversion that makes surface height or index variations directly detectable by an imaging system. The scaling surface mass sensitivity for the surface metrology application is S(scal) = 7 fg/mm under 40x magnification with a molecular resolution of approximately 12 IgG molecules within a pixel, limited by the surface roughness of the substrate. When applied to reverse-phase immunoassays in an antibody microarray format under 7x magnification, the current limit of detection is 10 ng/ml for 1 hour incubation, limited by biological and chemical variability. The biosensor is compatible with real-time binding measurements under active flow conditions with a binding dynamic range per well of 10(3) and a mass sensitivity of 2 pg/mm(2).
影响因子：
3.4
作者：
Kiessling, V;Tamm, LK
通讯作者：
Tamm, LK