Lipid Mass Tags via Aziridination for Probing Unsaturated Lipid Isomers and Accurate Relative Quantification**
Lipid Mass Tags via Aziridination for Probing Unsaturated Lipid Isomers and Accurate Relative Quantification**
复制标题
通过氮丙啶化的脂质质量标签用于探测不饱和脂质异构体和准确的相对定量**
DOI：
10.1002/anie.202207098
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yan, Xin
中科院分区：
文献类型：
--
作者：
Yang, Tingyuan;Tang, Shuli;Kuo, Syuan‐Ting;Freitas, Dallas;Edwards, Madison;Wang, Hongying;Sun, Yuxiang;Yan, Xin
了解脂质的浓度对于了解其生理功能和发现新的疾病生物标志物至关重要。然而，由于脂质的结构多样性和多种异构形式，准确定量脂质具有高度挑战性。为了解决这些关键差距，我们开发了一种新的基于氮丙啶的同量异序标签标记策略，该策略允许（i）测定脂质双键位置异构体，（ii）准确相对定量不饱和脂质，以及（iii）提高非极性脂质的电离效率。该方法的功效在表征和定量各种类别的脂质（如脂肪酸、磷酸甘油脂质、胆固醇酯（CE）和甘油酯）中得到了证明。在不使用脂质标准品的情况下，从阿尔茨海默病（AD）小鼠血清中同时鉴定和定量17种CE脂质异构体。其中，6种CE异构体在AD血清中的浓度变化显著。
Knowing concentrations of lipids is essential for understanding their physiological functions and discovering new disease biomarkers. However, it is highly challenging to accurately quantify lipids due to structural diversity and multiple isomeric forms of lipids. To address these critical gaps, we have developed a novel aziridine‐based isobaric tag labelling strategy that allows (i) determination of lipid double‐bond positional isomers, (ii) accurate relative quantification of unsaturated lipids, and (iii) improvement of ionization efficiencies of nonpolar lipids. The power of this method is demonstrated in characterization and quantification of various categories of lipids such as fatty acids, phosphoglycerol lipids, cholesteryl esters (CE), and glycerides. 17 CE lipid isomers were identified and quantified simultaneously from Alzheimer's disease (AD) mouse serum without using lipid standards. Among them, 6 CE isomers showed significant changes in concentrations in AD serum.