Homeostatic regulation of AMPA receptor expression at single hippocampal synapses
Homeostatic regulation of AMPA receptor expression at single hippocampal synapses
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DOI：
10.1073/pnas.0706447105
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发表时间：
2008-01-15
影响因子：
11.1
通讯作者：
Man, Heng-Ye
中科院分区：
文献类型：
--
作者：
Hou, Qingming;Zhang, Dawei;Man, Heng-Ye
稳态突触反应是将神经元活动限制在一个狭窄的生理范围内的重要手段。稳态可塑性是否表现出突触特异性，这是赫布型可塑性的一个关键特征，在很大程度上是未知的。在这里，我们报告说，在培养的海马神经元，α-氨基-3-羟基-5-甲基-异恶唑-4-丙酸亚型谷氨酸受体（AMPAR）的积累选择性增加慢性抑制单突触，而相邻的正常突触保持不受影响。这种突触特异性稳态调节依赖于突触活性的差异，并由GluR 2缺乏的AMPAR和PI 3激酶信号转导介导。这些结果表明突触特异性的存在和AMPAR门控钙离子在中枢神经元稳态可塑性中的关键作用。
Homeostatic synaptic response is an important measure in confining neuronal activity within a narrow physiological range. Whether or not homeostatic plasticity demonstrates synapse specificity, a key feature characteristic of Hebbian-type plasticity, is largely unknown. Here, we report that in cultured hippocampal neurons, alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid subtype glutamate receptor (AMPAR) accumulation is increased selectively in chronically inhibited single synapses, whereas the neighboring normal synapses remain unaffected. This synapse-specific homeostatic regulation depends on the disparity of synaptic activity and is mediated by GluR2-lacking AMPARs and PI3-kinase signaling. These results demonstrate the existence of synaptic specificity and the crucial role of AMPAR-gated calcium in homeostatic plasticity in central neurons.