Allergen Specific IgE Measurement with Polycheck Allergy: Comparison of Three Multiple Allergen Simultaneous Tests
Allergen Specific IgE Measurement with Polycheck Allergy: Comparison of Three Multiple Allergen Simultaneous Tests
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DOI：
10.3343/kjlm.2009.29.5.465
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发表时间：
2009-10-01
影响因子：
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通讯作者：
Kim, Jin Ju
中科院分区：
文献类型：
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作者：
Jang, Woo Ri;Nahm, Chung Hyun;Kim, Jin Ju
背景资料：体内皮肤点刺试验（SPT）或体外检测血清中过敏原特异性IgE常用于过敏性疾病的诊断。在这项研究中，我们评估了一种新的多种过敏原同时测试（MAST）免疫印迹试验，Polycheck过敏的有用性，（Biocheck GmbH，德国）。方法：通过SPT和三种不同的MAST测定法对总共100名具有过敏性疾病临床表现的患者进行测试：Polycheck Allergy（Biocheck GmbH，德国）、MAST CLA过敏系统（Hitachi Chemical Diagnostics，USA）和Allergy Screen（R-biopharm，德国）。结果：MAST法与SPT法的符合率Polycheck Allergy为79-100%，MAST CLA为88.9 ~ 100%，AllergyScreen为72.7-98.3%。在ROC曲线分析中，25种过敏原中有4种存在显著性差异：链格孢属、桦木属、榛子属和D。花粉对于链格孢属和桦树，Polycheck过敏（P
Background : The in vivo skin prick test (SPT) or in vitro detection of allergen specific IgE in serum is commonly used for the diagnosis of allergic disease. In this study, we evaluated the usefulness of a new multiple allergen simultaneous test (MAST) immunoblot assay, Polycheck Allergy (Biocheck GmbH, Germany).Methods: A total of 100 patients with clinical findings of allergic diseases were tested by SPT and three different MAST assays: Polycheck Allergy (Biocheck GmbH, Germany), MAST CLA allergy system (Hitachi Chemical Diagnostics, USA) and Allergy Screen (R-biopharm, Germany). The results of MAST assays were compared with those of SPT.Results: Concordance rates of MAST assays with SPT were 79-100% for Polycheck Allergy, 88.9-100% for MAST CLA and 72.7-98.3% for Allergy Screen. In ROC curve analysis, significant differences were observed in four of 25 allergens analysed: Alternaria, Birch, Hazelnut and D. farinae. For Alternaria and Birch, Polycheck Allergy (P