Accumulation and transport of cadmium by tolerant and susceptible strains of Mycobacterium scrofulaceum.
Accumulation and transport of cadmium by tolerant and susceptible strains of Mycobacterium scrofulaceum.
复制标题
瘰疬分枝杆菌耐受和敏感菌株对镉的积累和转运。
DOI：
10.1128/aac.33.3.350
复制
发表时间：
1989
影响因子：
4.9
通讯作者：
Falkinham3rd,JO
中科院分区：
文献类型：
--
作者：
Erardi,FX;Failla,ML;Falkinham3rd,JO
研究了两株对Cd2+敏感性不同的结核分枝杆菌对镉的积累和转运。10倍过量的Zn2+或Mn2+部分拮抗Cd2+对生长的抑制。耐和敏感菌株对cd2 +的吸收都与温度有关，并被10倍过量的Zn2+或Mn2+所抑制。耐药菌株和敏感菌株对109Cd2+的摄取动力学和累积的109Cd2+的保留量没有显著差异。耐受性菌株和易感菌株都能去除培养基中的大部分镉，但耐受性菌株的细胞能去除更多的镉。耐受性菌株积累的Cd2+大部分在颗粒部分，而不是在可溶性部分。细胞内积累的Cd2+主要集中在敏感菌株的可溶性部分。培养基中Cd2+的增加导致敏感菌株细胞中Mn2+和Zn2+含量降低，而耐受菌株细胞中Mn2+和Zn2+含量未降低。
Cadmium accumulation and transport were studied in two strains of Mycobacterium scrofulaceum differing in their susceptibility to Cd2+ toxicity. A 10-fold excess of either Zn2+ or Mn2+ partially antagonized inhibition of growth by Cd2+. 109Cd2+ uptake by both the tolerant and susceptible strains was temperature dependent and inhibited by a 10-fold excess of either Zn2+ or Mn2+. There were no significant differences in either the kinetics of 109Cd2+ uptake or the retention of accumulated 109Cd2+ by the tolerant and susceptible strains. Both tolerant and susceptible strains removed most of the cadmium from the culture medium, but significantly more was removed by cells of the tolerant strain. Most of the accumulated Cd2+ in the tolerant strain was in the particulate fraction, rather than in the soluble fraction. Intracellular accumulated Cd2+ was primarily in the soluble fraction of the susceptible strain. Increased Cd2+ in culture medium resulted in decreased Mn2+ and Zn2+ in cells of the susceptible strain but did not reduce the Mn2+ and Zn2+ content of cells of the tolerant strain.