A programmable microfluidic platform for multisample injection, discretization, and droplet manipulation
A programmable microfluidic platform for multisample injection, discretization, and droplet manipulation
复制标题
用于多样品进样、离散化和液滴操纵的可编程微流控平台
DOI：
10.1063/1.5143434
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发表时间：
2020-01-01
期刊：
影响因子：
3.2
通讯作者：
DeVoe, Don L.
中科院分区：
文献类型：
--
作者：
Babahosseini, Hesam;Padmanabhan, Supriya;DeVoe, Don L.
提出了一种可编程微流控平台，能够按需采样、分隔和操纵多个水溶液体积。该系统提供对微捕获器阵列的随机访问致动，支持从多个样本输入中选择性地离散皮升体积。该平台包括两个互连的芯片，在一个芯片内具有平行的T型结和多路微阀，使得能够可编程地注入含水样品塞，并且纳升体积被转移到第二个微捕集器阵列芯片，在该芯片中，塞被主动地离散为膜位移致动器静态阵列中的皮升液滴。该系统采用了两种不同的多路复用器设计，以减少样本注入和离散化所需的输入信号的数量。这项基于液滴的多功能技术为形成和操纵异质皮升液滴提供了灵活的样本工作流程和功能，特别适用于生化合成和需要并行和多步骤液滴过程的灵活和可编程操作的细胞分析应用。在这里，该平台用于在离散液滴阵列内选择性地封装差异标记细胞。
A programmable microfluidic platform enabling on-demand sampling, compartmentalization, and manipulation of multiple aqueous volumes is presented. The system provides random-access actuation of a microtrap array supporting selective discretization of picoliter volumes from multiple sample inputs. The platform comprises two interconnected chips, with parallel T-junctions and multiplexed microvalves within one chip enabling programmable injection of aqueous sample plugs, and nanoliter volumes transferred to a second microtrap array chip in which the plugs are actively discretized into picoliter droplets within a static array of membrane displacement actuators. The system employs two different multiplexer designs that reduce the number of input signals required for both sample injection and discretization. This versatile droplet-based technology offers flexible sample workflows and functionalities for the formation and manipulation of heterogeneous picoliter droplets, with particular utility for applications in biochemical synthesis and cell-based assays requiring flexible and programmable operation of parallel and multistep droplet processes. The platform is used here for the selective encapsulation of differentially labeled cells within a discrete droplet array.