BMI1 promotes the progression of laryngeal squamous cell carcinoma
BMI1 promotes the progression of laryngeal squamous cell carcinoma
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BMI1促进喉鳞状细胞癌的进展
DOI：
10.1016/j.oraloncology.2011.03.016
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发表时间：
2011-06-01
期刊：
影响因子：
4.8
通讯作者：
Tian, Jie
中科院分区：
文献类型：
--
作者：
Chen, Hui;Zhou, Liang;Tian, Jie
BMI1在多种恶性肿瘤中高表达，其表达水平与肿瘤进展、增殖和预后相关。然而，没有发表的研究检查BMI1在喉部鳞状细胞癌（SCC）中的作用。采用免疫荧光染色、实时荧光定量PCR和Western blotting分析BMI1在原发肿瘤中的表达。敲除BMI1，进行增殖、凋亡和细胞周期试验。对放化疗的敏感性进行了评估，并进行了体内致瘤性试验。BMI1在喉部SCCs中高表达。BMI1通过影响细胞周期，促进细胞增殖和肿瘤进展，抑制细胞凋亡。更重要的是，BMI1抑制喉部Hep2细胞对放化疗的敏化。BMI1对维持喉部SCCs的增殖和进展至关重要。因此，消耗BMI1可能是癌症管理的潜在治疗选择。(C) 2011 Elsevier Ltd.版权所有。
BMI1 is highly expressed in several malignant tumors, and its expression level is associated with tumor progression, proliferation, and prognosis. However, no published studies have examined the role of BMI1 in laryngeal squamous cell carcinoma (SCC).Expression of BMI1 in primary tumors was analyzed by immunofluorescence staining, real-time PCR, and Western blotting. BMI1 was knocked down, and proliferation, apoptosis, and cell cycle assays were performed. Sensitivity to radiochemotherapy was evaluated, and tumorigenicity assays were performed in vivo.BMI1 was highly expressed in laryngeal SCCs. BMI1 promoted cell proliferation and tumor progression, and inhibited apoptosis due to influences on the cell cycle. More importantly, BMI1 suppressed the sensitization of laryngeal Hep2 cells to radiochemotherapy.BMI1 is essential to maintain the proliferation and progression of laryngeal SCCs. Therefore, depletion of BMI1 may be a potential therapeutic option for cancer management. (C) 2011 Elsevier Ltd. All rights reserved.