Integrin αvβ3 promotes infection by Japanese encephalitis virus
Integrin αvβ3 promotes infection by Japanese encephalitis virus
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整合素αvβ3促进日本脑炎病毒感染
DOI：
10.1016/j.rvsc.2016.12.007
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发表时间：
2017-04-01
影响因子：
2.4
通讯作者：
Li, Xiangmin
中科院分区：
文献类型：
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作者：
Fan, Wenchun;Qian, Ping;Li, Xiangmin
日本脑炎病毒（JEV）是一种蚊媒黄病毒，是全球病毒性脑炎疾病的主要病因之一。JEV包膜蛋白促进病毒进入，其结构域III包含一个Arg-Gly-Asp （RGD）基序，该基序可能通过RGD结合整合素调节JEV进入。本研究评价了整合素α v和β 3在乙脑病毒感染中的作用。通过特殊shRNA降低整合素α v/ β 3的表达，可以在BHK-21细胞中抑制2至4倍的乙脑病毒复制。同时，整合素α v/ β 3特异性抗体对乙脑病毒传染性的抑制作用分别为58%和33%，rgd特异性肽的抑制作用为36%。转染编码人整合素β 3的cDNA后，CHO细胞的E蛋白表达和JEV RNA载量明显增加。此外，整合素在病毒结合阶段介导乙脑病毒感染。因此，我们的研究表明，整合素av和β 3是与乙脑病毒进入靶细胞相关的宿主因子。(C) 2016 Elsevier Ltd.版权所有。
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that is one of the major causes of viral encephalitis diseases worldwide. The JEV envelope protein facilitates viral entry, and its domain III contains an Arg-Gly-Asp (RGD) motif, that may modulate JEV entry through the RGD-binding integrin. In this study, the roles of integrin alpha v and beta 3 on the infection of JEV were evaluated. Reduced expression of integrin alpha v/beta 3 by special shRNA confers 2 to 4-fold inhibition of JEV replication in BHK-21 cells. Meanwhile, antibodies specific for integrin alpha v/beta 3 displayed similar to 58% and similar to 33% inhibition of JEV infectivity and RGD-specific peptides produced 36% of inhibition. Expression of E protein and JEV RNA loads were clearly increased in CHO cells transfected with cDNA encoding human integrin beta 3. Moreover, integrin av mediates JEV infection in viral binding stage of life cycle. Therefore, our study suggested that integrin av and beta 3 serve as a host factor associated with JEV entry into the target cells. (C) 2016 Elsevier Ltd. All rights reserved.