Effects of hexanoic acid on microbial communities, fermentation, and hygienic quality of corn silages infested with toxigenic fungi
Effects of hexanoic acid on microbial communities, fermentation, and hygienic quality of corn silages infested with toxigenic fungi
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己酸对产毒真菌侵染玉米青贮饲料微生物群落、发酵及卫生品质的影响
DOI：
10.1002/jsfa.11696
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发表时间：
2021
影响因子：
4.1
通讯作者：
Xianjun Yuan
中科院分区：
文献类型：
--
作者：
Wenbo Wang;Xin Yang;Junfeng Li;Zhihao Dong;Jie Zhao;Tao Shao;Xianjun Yuan
本研究旨在揭示己酸对有无真菌感染的玉米青贮微生物群落、发酵和卫生质量的影响。将真菌侵染（FI）和非真菌侵染（NFI）的整株玉米样品分别在不含（对照，CON）或含己酸（Hex，90%纯度）的情况下以0.2 g·kg− 1鲜重（FW）进行青贮。在45天的青贮期间，Hex青贮饲料中的乳酸（LA）浓度低于CON中的LA浓度;然而，在第90天，FI-Hex青贮饲料在处理中具有最高的LA浓度。六角青贮饲料显示较低的黄曲霉毒素B1（AFB 1），玉米赤霉烯酮（ZEA），脱氧雪腐镰刀菌烯醇（DON）浓度比CON FI青贮饲料。在第5天，与CON相比，添加Hex降低了克雷伯氏菌、泛菌和肠球菌的相对丰度，而与真菌感染无关。这种抑制作用持续到第90天的NFI青贮饲料，但消失的FI青贮饲料在第90天。真菌感染导致念珠菌（34.05%）和Wickerhamomyces（19.46%）的积累。Hex降低了NFI青贮料第5天的青霉菌、伊萨酵母菌和青霉菌的相对丰度;然而，在FI青贮料第5天没有观察到其抑制作用。结论真菌感染与玉米青贮料的发酵和卫生质量差有关。添加Hex可加速pH值下降，并保持抑菌活性至青贮90 d，减轻真菌侵染对发酵的不利影响，防止霉菌毒素在玉米青贮中的积累。© 2021化学工业协会。
BACKGROUNDThis study aims to reveal the effects of hexanoic acid on the microbial communities, fermentation, and the hygienic quality of corn silages with or without fungal infection. Fungal‐infested (FI) and non‐infested (NFI) whole‐crop corn samples were separately ensiled without (control, CON) or with hexanoic acid (Hex, 90% purity) at 0.2 g·kg−1fresh weight (FW).RESULTThe addition of Hex accelerated the pH decline during the first 5 days of ensiling regardless of fungal infestation. The lactic acid (LA) concentration in Hex silages was lower than that in CON during 45 days of ensiling; however the FI‐Hex silage had the highest LA concentration among treatments on day 90. The Hex silage showed lower aflatoxin B1(AFB1), zearalenone (ZEA), and deoxynivalenol (DON) concentrations than CON for FI silages. On day 5, the addition of Hex decreased the relative abundance ofKlebsiella,Pantoea, andEnterobactercompared with CON, regardless of fungal infestation. This inhibitory effect lasted until day 90 for NFI silages but disappeared for FI silages on day 90. The fungal infestation resulted in the accumulation ofCandida(34.05%) andWickerhamomyces(19.46%). Hex decreased the relative abundance ofAsperigillus,Issatchenkia,andPenicilliumfor NFI silages on day 5; however, its inhibitory effects were not observed in FI silages on day 5.CONCLUSIONFungal infestation was associated with poor fermentation and hygienic quality of corn silage. Adding Hex accelerated the pH decline and maintained the antifungal activity until 90 days of ensiling, attenuating adverse effects of fungal infestation on the fermentation and preventing the accumulation of mycotoxins in corn silages. © 2021 Society of Chemical Industry.