Longitudinal transcriptomic characterization of viral genes in HSV-1 infected tree shrew trigeminal ganglia
Longitudinal transcriptomic characterization of viral genes in HSV-1 infected tree shrew trigeminal ganglia
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HSV-1感染树鼩三叉神经节病毒基因的纵向转录组特征
DOI：
10.1186/s12985-020-01344-8
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发表时间：
2020-07-08
期刊：
影响因子：
4.8
通讯作者：
Zhou, Jumin
中科院分区：
文献类型：
--
作者：
Wang, Erlin;Ye, Yunshuang;Zhou, Jumin
单纯疱疹病毒1型（HSV-1）急性感染后，在三叉神经节（TG）感觉神经元中建立终身潜伏期和反复激活。受感染的树鼩与小鼠不同，表现出与人类感染相似的特征。一个详细的转录组学分析的树模型可以提供机制的见解HSV-1 infectioninhuman.MethodsWe测序的转录组感染的TG从树和小鼠，和4个人类供体，然后检查病毒基因表达达58天感染TG从小鼠和树，并比较潜伏期数据与人类TG。我们发现，在急性感染期间，所有HSV-1基因在小鼠TG中均能检测到，但在该阶段，22个病毒转录、复制和病毒成熟所必需的病毒基因在树鼩 TG中不表达。重要的是，在潜伏期期间，我们发现LAT可以在小鼠和树鼩中检测到，但后者也具有小鼠中不存在但在人类样品中存在的ICP 0转录物信号。重要的是，我们观察到感染的人和树的TG具有更相似的LAT区域转录峰。更重要的是，我们观察到，HSV-1自发reactivates潜伏感染的树shrews具有相对较高的efficiency.ConclusionsThese结果代表了第一个纵向转录组表征HSV-1感染的急性，潜伏期和复发阶段，并透露，树shrew感染具有重要的相似功能与人类感染。
BackgroundFollowing acute infection, Herpes Simplex virus-1 (HSV-1) establishes lifelong latency and recurrent reactivation in the sensory neurons of trigeminal ganglia (TG). Infected tree shrew differs from mouse and show characteristics similar to human infection. A detailed transcriptomic analysis of the tree shrew model could provide mechanistic insights into HSV-1 infection in humans.MethodsWe sequenced the transcriptome of infected TGs from tree shrews and mice, and 4 human donors, then examined viral genes expression up to 58 days in infected TGs from mouse and tree shrew, and compare the latency data with that in human TGs.ResultsHere, we found that all HSV-1 genes could be detected in mouse TGs during acute infection, but 22 viral genes necessary for viral transcription, replication and viral maturation were not expressed in tree shrew TGs during this stage. Importantly, during latency, we found that LAT could be detected both in mouse and tree shrew, but the latter also has an ICP0 transcript signal absent in mouse but present in human samples. Importantly, we observed that infected human and tree shrew TGs have a more similar LAT region transcription peak. More importantly, we observed that HSV-1 spontaneously reactivates from latently infected tree shrews with relatively high efficiency.ConclusionsThese results represent the first longitudinal transcriptomic characterization of HSV-1 infection in during acute, latency and recurrent phases, and revealed that tree shrew infection has important similar features with human infection.