Comparative evaluation of antioxidant potential of alaternin (2-hydroxyemodin) and emodin
Comparative evaluation of antioxidant potential of alaternin (2-hydroxyemodin) and emodin
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DOI：
10.1021/jf000936r
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发表时间：
2000-12-01
影响因子：
6.1
通讯作者：
Yokozawa, T
中科院分区：
文献类型：
--
作者：
Choi, JS;Chung, HY;Yokozawa, T
比较了大黄素（2-羟基大黄素）和大黄素的抗氧化活性，分别用硫氰酸盐法抑制亚油酸体系中的脂质过氧化，用2',7'-二氯二氢荧光素双乙酸酯法抑制肾脏匀浆中总活性氧的生成，用3- morpholinosydnon亚胺体系抑制生成超氧自由基和一氧化氮的过氧亚硝酸盐的生成，以及清除真正的过氧亚硝酸盐。硫氰酸盐法研究发现，黄酮素和大黄素均能抑制亚油酸的过氧化，且呈剂量依赖性。前者在活性氧和氮介导的反应中表现出抑制活性，后者则没有。这些结果表明，alaternin是一种潜在有效的多功能抗氧化剂，可用于保护生物系统和功能免受各种氧化应激。
The antioxidant activities of alaternin (2-hydroxyemodin) and emodin were compared for their respective potentials to inhibit lipid peroxidation in the linoleic acid system by the thiocyanate method, to inhibit total reactive oxygen species generation in kidney homogenates using 2',7'-dichlorodihydrofluorescein diacetate, to inhibit peroxynitrite formation by the 3-morpholinosydnonimine system, which generates superoxide radical and nitrogen monooxide, and to scavenge authentic peroxynitrites. Both alaternin and emodin were found to inhibit the peroxidation of linoleic acid by the thiocyanate method in a dose-dependent manner. Whereas the former shows inhibitory activities in reactive oxygen- and nitogen-mediated reactions, the latter does not. These results indicate that alaternin is a potentially effective and versatile antioxidant and can be used to protect biological systems and functions against various oxidative stresses.