Plasma ghrelin isoforms and gastric ghrelin O-acyltransferase expression are influenced by Helicobacter pylori status
Plasma ghrelin isoforms and gastric ghrelin O-acyltransferase expression are influenced by Helicobacter pylori status
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DOI：
10.1016/j.nut.2011.11.023
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发表时间：
2012-10-01
期刊：
影响因子：
4.4
通讯作者：
Inui, Akio
中科院分区：
文献类型：
--
作者：
Ando, Takashi;Mizuno, Shigeto;Inui, Akio
目的：幽门螺杆菌可影响宿主的营养状态。本研究旨在探讨幽门螺杆菌感染状态与胃肠促生长素系统动态变化的关系。方法：对30名受试者进行临床研究，主要集中在以下几点：1)幽门螺杆菌感染对胃粘膜循环中胃促生长素亚型浓度的影响；2)根除幽门螺杆菌对上述参数的影响。结果：16例幽门螺杆菌阳性患者的血浆胃促生长素和去脂酰胃促生长素浓度均显著低于14H组。幽门螺杆菌阴性对照。Hp阳性组和Hp阴性组的酰基-ghrelin/去酰基-ghrelin比值无显著差异。幽门螺杆菌阳性组胃粘膜中Ghrelin和山羊基因的表达水平显著低于阴性组。在根除Hp成功的11例受试者中，根除Hp后血浆去脂酰-ghrelin水平有升高趋势，但差异不显著，但血浆去酰基-ghrelin水平显著降低。虽然根除幽门螺杆菌后胃Ghrelin基因表达显著增加，但胃内Ghrelin基因表达有增加趋势，但变化不明显。结论：幽门螺杆菌感染状态可能通过改变血浆Ghrelin亚型水平以及胃内Ghrelin和山羊基因表达水平来影响宿主的营养状况。(C)2012 Elsevier Inc.保留所有权利。
Objective: Helicobacter pylori is known to affect the host's nutritional status. This study was performed to elucidate the relationship between H. pylori status and the dynamics of the ghrelin system, in the context of ghrelin O-acyltransferase (GOAT) expression.Methods: We conducted a clinical study of 30 subjects focusing on the following points: 1) the effects of H. pylori infection on the concentrations of circulating ghrelin isoforms and on ghrelin and GOAT mRNA expression in the gastric mucosa, and 2) the effects of H. pylori eradication on the same parameters.Results: The plasma acyl-ghrelin and desacyl-ghrelin concentrations of 16 H. pylori positive participants were significantly lower than those of 14H. pylori negative controls. The acyl-ghrelin/desacyl-ghrelin ratio was not significantly different between the H. pylori positive and H. pylori negative participants. The levels of ghrelin and GOAT mRNA in the gastric mucosa were significantly lower in the H. pylori positive participants than in the H. pylori negative controls. In 11 subjects in whom H. pylori eradication was successful, their plasma acyl-ghrelin levels tended to increase after H. pylori eradication, but the difference was not significant; however, their plasma desacyl-ghrelin levels were significantly reduced. Although gastric ghrelin mRNA expression increased significantly after H. pylori eradication, gastric GOAT mRNA expression tended to increase but was not significantly altered.Conclusion: H. pylori status might affect the host's nutritional status through changes in the plasma levels of ghrelin isoforms and the gastric expression levels of ghrelin and GOAT mRNA. (C) 2012 Elsevier Inc. All rights reserved.