BRONCHOALVEOLAR LAVAGE FLUID PROTEINS IN HUMAN LUNG-DISEASE - ANALYSIS BY 2-DIMENSIONAL ELECTROPHORESIS
BRONCHOALVEOLAR LAVAGE FLUID PROTEINS IN HUMAN LUNG-DISEASE - ANALYSIS BY 2-DIMENSIONAL ELECTROPHORESIS
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DOI：
10.1002/elps.1150140141
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发表时间：
1993-03-01
期刊：
影响因子：
2.9
通讯作者：
MAIER, K
中科院分区：
文献类型：
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作者：
LENZ, AG;MEYER, B;MAIER, K
用磷酸盐缓冲盐水冲洗肺上皮衬里液获得人支气管肺泡灌洗液的蛋白质，在变性和还原条件下通过二维电泳进行分析。将健康志愿者（对照组）的支气管肺泡灌洗液蛋白的二维模式与特发性肺纤维化、结节病和石棉肺患者的支气管肺泡灌洗液蛋白的二维模式进行比较。人们特别关注主要存在于凝胶低分子量区域的少量蛋白质。观察到单个蛋白质点的显着变化。在特发性肺纤维化中，表面活性剂相关蛋白 SP-A 的斑点强度显着降低，SP-A 在电荷和分子量方面均显示异构体形式。结节病时，免疫球蛋白（IgG、IgA）和一组等电点为4.5-5.0、分子量为55 kDa的蛋白点增加。另一个点出现在等电点为 4.5、分子量为 12 kDa 处。特别是在石棉沉滞症中，而且在某些特发性肺纤维化和结节病病例中，低分子量蛋白质的数量和强度强烈增加。
Proteins of human bronchoalveolar lavage fluids, obtained by washing the epithelial lining fluid of the lungs with phosphate-buffered saline, were analyzed by two-dimensional electrophoresis under denaturating and reducing conditions. The two-dimensional pattern of bronchoalveolar lavage fluid proteins of healthy volunteers (controls) were compared with those of patients with idiopathic pulmonary fibrosis, sarcoidosis, and asbestosis. Particular interest was paid to the proteins present in minor amounts mainly in the low molecular weight region of the gels. Marked changes in single protein spots were observed. In idiopathic pulmonary fibrosis the spot intensity of the surfactant-associated protein, SP-A, showing isomeric forms both in charge and in molecular weight, was markedly decreased. In sarcoidosis, the immunoglobulins (IgG, IgA) and a group of protein spots at an isoelectric point of 4.5-5.0 and a molecular mass of 55 kDa were increased. An additional spot appeared at an isoelectric point of 4.5 and a molecular mass of 12 kDa. In particular in asbestosis, but also in some cases of idiopathic pulmonary fibrosis and sarcoidosis, the number and intensity of low molecular weight proteins were increased strongly.