Adhesion molecules, extracellular matrix, and proteases in prostate carcinoma.
Adhesion molecules, extracellular matrix, and proteases in prostate carcinoma.
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前列腺癌中的粘附分子、细胞外基质和蛋白酶。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
A. Cress
中科院分区：
文献类型：
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作者：
Raymond B. Nagle;J. Knox;C. Wolf;G. Tim Bowden;A. Cress
前列腺癌的免疫组化研究表明，大多数原发性癌，包括高级别肿瘤，被一个由层粘连蛋白，IV型胶原和巢蛋白组成的基底层包围。除了预期的层粘连蛋白亚链A、B1、B2外，还发现了亚链M和S。正常腺体的基底细胞表达几种整合素α单位，包括α 2、3、4、5、6和V。观察到β 1和β 4亚单位。这些整合素单位在细胞的基部极化，在那里它们与周围的细胞外基质共分布。整合素α 6 β 4与半桥粒样结构相关，如通过透射电子显微镜（TEM）检测到的。在癌中，未观察到β 4亚基，α 6和β 1亚基呈双表达。整合素在癌中的表达是弥漫性的，在细胞质膜中，而不限于细胞的基底面。此外，与半桥粒相关的VII型胶原蛋白和BP 180蛋白丢失，尽管BP 230、网蛋白和HD 1蛋白不表达。采用免疫组化和北方分析，我们观察到三种金属蛋白酶在前列腺癌中的表达--基质溶解素、明胶酶A和明胶酶B。蛋白质印迹和酶谱分析表明，在这三者中，只有基质溶解素似乎以其活性形式存在。最近的原位杂交研究显示，在25/33原发癌中的基质溶解素mRNA的局部表达。基质溶素也似乎在前列腺导管和萎缩腺体中高度表达。三种金属蛋白酶的表达也见于前列腺上皮内瘤变病变。（250字处删节）
Immunohistochemical studies of prostate carcinoma reveal that most primary carcinomas, including high-grade tumors, are surrounded by a basal lamina composed of laminin, type IV collagen, and entactin. In addition to the expected laminin subchains A, B1, B2, subchains M and S are also found. Tenascin, found around normal glands, is seen in 60% of carcinomas. The basal cells of the normal gland express several integrin alpha units including alpha 2,3,4,5,6, and v. Both beta 1 and beta 4 subunits are observed. These integrin units are polarized at the base of the cells where they codistribute with the surrounding extracellular matrix. The integrin alpha 6 beta 4 is associated with hemidesmosomal-like structures, as detected by transmission electron microscopy (TEM). In carcinoma, the beta 4 is not observed and the alpha 6 and beta 1 subunits are variably expressed. The integrin expression in carcinoma is diffuse in the cytoplasmic membrane and not restricted to the basal aspects of the cell. In addition, type VII collagen and the BP 180 protein which are associated with hemidesmosomes are lost, although the BP 230, plectin, and HD1 proteins are variably expressed. Using immunohistochemistry and northern analysis we observed three metalloproteinases in prostate carcinoma--matrilysin, gelatinase A, and gelatinase B. Western blotting and zymogram analysis reveal that of these three, only matrilysin appears to be present in its active form. Recent in situ hybridization studies reveal focal expression of the matrilysin mRNA in 25/33 primary carcinomas. Matrilysin also appears to be highly expressed in prostatic ducts and atrophic glands. Expression of the three metalloproteinases is also seen in prostatic intraepithelial neoplasia lesions.(ABSTRACT TRUNCATED AT 250 WORDS)