Hydrogen-rich solution attenuates cold ischemia-reperfusion injury in rat liver transplantation
Hydrogen-rich solution attenuates cold ischemia-reperfusion injury in rat liver transplantation
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DOI：
10.1186/s12876-019-0939-7
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发表时间：
2019-02-08
影响因子：
2.4
通讯作者：
Hibi, Taizo
中科院分区：
文献类型：
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作者：
Uto, Keiichi;Sakamoto, Seisuke;Hibi, Taizo
背景肝移植（LT）被认为是终末期肝病的标准治疗方法，但理想的供体仍然有限，导致不可避免地增加了对边缘供体移植物的需求。因此，在这种边缘供体中减轻缺血再灌注损伤（IRI）对于降低移植物原发性无功能和移植物丢失的可能性至关重要。有报道发现，分子氢在一些非肝移植模型中显示出抗氧化和抗炎作用，可预防IRI。因此，我们调查是否分子氢可以减弱IRI在LT model rats.MethodsWe使用了富氢水浴溶解氢到溶液和移植组织，并进行同基因和原位LT在刘易斯大鼠与威斯康星州大学（UW）的解决方案。再灌注6 h后取血和组织标本。测定血清肝酶。病理结果包括细胞因子和血红素氧合酶（HO）-1在肝组织中的表达进行了evaluated.ResultsThe内的氢浓度的移植组织增加，取决于存储时间，1h后平台。与对照组相比，给予富氢UW溶液保存的移植物组的血清肝酶水平显著降低，肝损伤的组织学评分显著减轻。与对照组相比，富氢UW液组氧化损伤和肝细胞凋亡明显减轻，促炎细胞因子表达降低，HO-1蛋白水平明显升高（n=3-12/组，P
BackgroundLiver transplantation (LT) is considered the standard treatment for end-stage liver disease, but ideal donors remain in limited supply, resulting in an unavoidable increase in the need to use grafts from marginal donors. The attenuation of ischemia-reperfusion injury (IRI) in such marginal donors is therefore crucial for reducing the possibility of the primary non-function of grafts and graft loss. Some reports have found that molecular-hydrogen showed antioxidant and anti-inflammatory effects in preventing IRI in some non-hepatic transplant models. Therefore, we investigated whether or not molecular-hydrogen could attenuate IRI in LT model rats.MethodsWe used a hydrogen-rich water bath to dissolve hydrogen into solution and graft tissues and performed isogenic and orthotopic LT in Lewis rats with University of Wisconsin (UW) solution. Blood and tissue samples were collected 6h after the reperfusion. Hepatic enzymes in serum were measured. Pathological findings including the expressions of cytokines and heme oxygenase (HO)-1 in liver tissues were evaluated.ResultsThe concentration of hydrogen inside the graft tissues increased depending on the storage time, plateauing after 1h. Serum liver enzyme levels were significantly lower and the histology score of liver damage markedly attenuated in the group given grafts preserved in hydrogen-rich UW solution than in the control group. The hydrogen-rich UW solution group also showed less oxidative damage and hepatocyte apoptosis than the control group, and the expression of proinflammatory cytokines tended to be lower while the protein levels of HO-1 were significantly increased (n=3-12 per group, P