Promotion of enamel caries remineralization by an amelogenin-derived peptide in a rat model
Promotion of enamel caries remineralization by an amelogenin-derived peptide in a rat model
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牙釉蛋白衍生肽在大鼠模型中促进牙釉质龋齿再矿化
DOI：
10.1016/j.archoralbio.2016.09.009
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发表时间：
2017-01-01
影响因子：
3
通讯作者：
Zhang, Linglin
中科院分区：
文献类型：
--
作者：
Han, Sili;Fan, Yingying;Zhang, Linglin
目的：在由pH循环诱导的初始龋齿的体外模型中，已显示釉原蛋白衍生肽促进脱矿釉质的再矿化。本研究探讨了肽是否在体内复杂的口腔环境中发挥类似的作用。设计：无特定病原体的Sprague-Dawley大鼠（n =36）感染变形链球菌，自由进食饮食2000和补充蔗糖的饮用水（10%，w/v），然后随机分为三组，分别用25 μ M肽溶液、1 g/L NaF或去离子水处理。每天用相应的溶液擦拭臼齿两次，持续24天。然后处死动物，取下颌骨，用定量光诱导荧光数字（QLF-D）技术分析龋齿病变，以测量矿物质含量的变化。为了验证QLF-D结果，使用Keyes方法对龋损深度和大小进行评分，并使用偏振光显微镜（PLM）进行分析。结果：基于QLF-D结果（Δ F和Δ Q），用肽或NaF处理的牙齿中的矿物质获得显著高于用水处理的牙齿（p < 0.05）。根据Keyes评分，用肽或NaF处理的大鼠的光滑表面龋和沟龋的发生率相似，并且在这些组中显著低于用水处理的大鼠（p < 0.05）。基于PLM，用肽或NaF治疗的牙齿上的病变较浅。没有观察到显着差异之间的磨牙釉质龋治疗肽或NaF.Conclusions：这种釉原蛋白衍生的肽可以促进在大鼠龋齿模型中的矿化，表明具有很强的潜力，为临床使用。(C)2016爱思唯尔有限公司版权所有。
Objective: An amelogenin-derived peptide has been shown to promote remineralization of demineralized enamel in an in vitro model of initial caries induced by pH cycling. The present study examines whether the peptide exerts similar effects within the complex oral environment in vivo.Design: Specific pathogen-free Sprague-Dawley rats (n =36) were infected with Streptococcus mutans, given ad libitum access to Diet 2000 and drinking water supplemented with sucrose (10%, w/v), and then randomly divided into three groups treated with 25 mu M peptide solution, 1 g/L NaF or deionized water. Molar teeth were swabbed twice daily with the respective solutions for 24 days. Then animals were killed, their jaws were removed and caries lesions were analyzed using the quantitative light-induced fluorescence-digital (QLF-D) technique to measure changes in mineral content. To verify QLF-D results, caries were scored for lesion depth and size using the Keyes method, and analyzed using polarized light microscopy (PLM).Results: Mineral gain was significantly higher in teeth treated with peptide or NaF than in teeth treated with water (p < 0.05), based on the QLF-D results (Delta F and Delta Q). Incidence of smooth-surface and sulcal caries based on Keyes scores was similar in rats treated with peptide or NaF, and significantly lower in these groups than in rats treated with water (p < 0.05). Lesions on teeth treated with peptide or NaF were shallower, based on PLM. No significant differences were observed between molar enamel caries treated with peptide or NaF.Conclusions: This amelogenin-derived peptide can promote remineralization in a rat caries model, indicating strong potential for clinical use. (C) 2016 Elsevier Ltd. All rights reserved.