Antibody-independent, CD4+ T-Cell-Dependent protection against pneumococcal colonization elicited by intranasal immunization with purified pneumococcal proteins
Antibody-independent, CD4+ T-Cell-Dependent protection against pneumococcal colonization elicited by intranasal immunization with purified pneumococcal proteins
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DOI：
10.1128/iai.00773-07
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发表时间：
2007-11-01
影响因子：
3.1
通讯作者：
Malley, Richard
中科院分区：
文献类型：
--
作者：
Basset, Alan;Thompson, Claudette M.;Malley, Richard
通过暴露于活的或灭活的肺炎球菌，小鼠对肺炎球菌定植的免疫已被证明是抗体独立的，但依赖于CD 4（+）T细胞。在这里，我们表明，鼻内免疫与肺炎球菌蛋白（肺炎球菌表面蛋白C，粘附素A，和pneumolysoid）可以引起类似的保护机制。在用这种蛋白质混合物鼻内免疫后，免疫球蛋白先天缺陷的小鼠中的定殖可以显著减少;相反，在攻击时免疫的野生型小鼠中CD 4（+）T细胞的耗竭消除了免疫提供的保护。总之，我们的研究结果表明，鼻内免疫与肺炎球菌蛋白的混合物保护抗体不依赖，CD 4（+）T细胞依赖的方式对殖民。
Immunity to pneumococcal colonization in mice by exposure to live or killed pneumococci has been shown to be antibody independent but dependent on CD4(+) T cells. Here we show that intranasal immunization with pneumococcal proteins (pneumococcal surface protein C, adhesin A, and a pneumolysoid) can elicit a similar mechanism of protection. Colonization could be significantly reduced in mice congenitally deficient in immunoglobulins after intranasal immunization with this mixture of proteins; conversely, the depletion of CD4(+) T cells in immunized wild-type mice at the time of challenge eliminated the protection afforded by immunization. Overall, our results show that intranasal immunization with a mixture of pneumococcal proteins protects against colonization in an antibody-independent, CD4(+) T-cell-dependent manner.