Peripheral Nerve Repair: Monitoring by Using Gadofluorine M-enhanced MR Imaging with Chitosan Nerve Conduits with Cultured Mesenchymal Stem Cells in Rat Model of Neurotmesis
Peripheral Nerve Repair: Monitoring by Using Gadofluorine M-enhanced MR Imaging with Chitosan Nerve Conduits with Cultured Mesenchymal Stem Cells in Rat Model of Neurotmesis
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DOI：
10.1148/radiol.11110911
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发表时间：
2012-01-01
期刊：
影响因子：
19.7
通讯作者：
Shen, Jun
中科院分区：
文献类型：
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作者：
Liao, Cheng-De;Zhang, Fang;Shen, Jun
目的：应用磁共振成像技术观察组织工程支架植入后大鼠神经修复的纵向变化，并确定是否可以用加多夫林M增强磁共振成像或神经T2松弛时间测量来监测组织工程支架促进神经再生的情况。材料和方法：所有实验方案均经机构动物使用和护理委员会批准。将间充质干细胞(MSCs)种植到壳聚糖神经管中，制备组织工程化支架。36只大鼠坐骨神经横断伤后植入神经管，植入MSC或不植入MSC。在8周的随访期内进行连续T2测量、加多夫林M增强磁共振成像和坐骨神经功能指数测量，并定期进行组织学评估。结果：神经T2松弛时间、Gadofl uorine M增强T1加权像信号强度与神经T2松弛时间、Gadofl uorine M增强及功能改变具有相似的时间进程。与单纯植入壳聚糖管的神经相比，植入MSC管的神经功能恢复稍好，神经再生增强，T2松弛时间恢复较慢，Gadofloorine M增强较快下降。与加多夫林M增强相比，切断神经远端的T2值恢复到基线水平的速度更快。结论：利用加多夫林M增强磁共振成像和T2松弛时间测量可以监测组织工程神经修复周围神经。T2弛豫时间在检测神经再生方面似乎比Gadofl uorine M增强MRI更敏感。(C)RSNA，2011年
Purpose:To observe the longitudinal changes of nerve repair in rats after tissue-engineered construct implantation at magnetic resonance (MR) imaging and to determine whether the enhanced nerve regeneration with use of tissue-engineered constructs could be monitored with gadofl uorine M-enhanced MR imaging or nerve T2 relaxation time measurement.Materials and Methods:All experimental protocols were approved by the institutional Animal Use and Care Committee. Tissue-engineered constructs were prepared by seeding mesenchymal stem cells (MSCs) into chitosan nerve tubes. Thirty-six rats with sciatic nerve transection injury underwent nerve tube implantation with (n = 18) or without (n = 18) MSC seeding. Sequential T2 measurement, gadofl uorine M-enhanced MR imaging, and sciatic function index measurement were performed over an 8-week follow-up period, with histologic assessments performed at regular intervals. T2 relaxation times and signal intensity at gadofl uorine M-enhanced T1-weighted imaging were measured and were compared by using repeated-measures analysis of variance followed by the Student-Neuman-Keuls post-hoc test for multiple pairwise comparisons.Results:Nerve T2 relaxation times and gadofl uorine M enhancement, as well as functional changes, showed a similar time course. Nerves implanted with MSC-seeded tubes achieved slightly better functional recovery and enhanced nerve regeneration while showing a slower return to baseline T2 relaxation time and a more rapid decline in gadofl uorine M enhancement compared with nerves implanted with chitosan tubes alone. T2 values of the distal portion of transected nerves showed a more rapid return to baseline level than did gadofl uorine M enhancement.Conclusion:Peripheral nerve repair with use of tissue-engineered constructs can be monitored by using gadofl uorine M-enhanced MR imaging and T2 relaxation time measurements. T2 relaxation time seems more sensitive than gadofl uorine M-enhanced MR imaging for detecting nerve regeneration. (C) RSNA, 2011