Mesenteric ischemia/reperfusion-induced intestinal and vascular damage:: Effect of stobadine
Mesenteric ischemia/reperfusion-induced intestinal and vascular damage:: Effect of stobadine
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DOI：
10.1358/mf.2007.29.1.1063495
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Sotnikova, R.
中科院分区：
文献类型：
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作者：
Nosal'ova, V.;Navarova, J.;Sotnikova, R.
本研究观察了吡哆醇类化合物舒拉定对大鼠脑缺血再灌注后血管损伤的影响。结扎肠系膜上动脉(SMA)60min后再灌流30min，造成缺血模型。测定GSH含量、溶酶体酶N-乙酰-β-D-葡萄糖苷酸酶活性和γ-谷氨酰转肽酶活性等生化指标，以确定肠道损伤程度。血管I/R损伤评价为等长条件下乙酰胆碱引起的肠系膜动脉环松弛的变化。用放大化学发光法(CL)检测活性氧(ROS)的产生。IIR后可见不同程度的肠损伤，以回肠末端损伤最明显。IIR的作用主要表现为血管通透性增加，蛋白渗漏和随后的肠出血损伤，以及内皮依赖性SMA松弛受损。血管功能障碍表现为对乙酰胆碱的最大松弛反应降低。在肠道和血管组织中都观察到CL的增加，这表明ROS的产生增加。一种新的抗氧化剂司他丁被发现可以降低I/R引起的血管通透性增加和小肠损伤的程度，改善伴随I/R的生化变化，保护血管内皮依赖性的系膜动脉松弛，并减弱CL反应。司多巴丁的有益作用提示它可能用于预防和/或治疗I/R所致的病理改变。(C)2007年Pek Science。版权所有。
This study examined the effects of the pyridoindole compound slobadine and vascular injury following messentric ischemical reperfusion (I/R) in rats. Ischemia was induced by occlusion of the superior mesentric artery (SMA) for 60 min followed by 30 min reperfusion. To characterize gut impairment gut impairment , some parameters of intestinal damage and biochemical variables such as GSH content, activity of a lysosomal enzyme N-acetyl-beta-D-glucuronidase and activity of gamma-glutamyl transpeptidase were determined. Vascular I/R-induced damage was evaluated as changes in acetylcholine evoked relaxation of mesenteric artery rings under isometric conditions. A method of amplied chemiluminescence (CL) was used to detect production of reactive oxygen species (ROS). Following IIR, pronounced intestinal injury of various intensities was observed, with maximal changes occurring in the terminal ileum. The effect of IIR was expressed mainly as increased vascular permeability, with protein leakage and subsequent hemorrhagic injury of the intestine as well as impaired endothelium-dependant SMA relaxation. Vessel dysfunction was manifested by a decrease of the maximal relaxation response to acetylcholine. An increase of CL, indicative of increased ROS production, was observed in both intestinal and vascular tissue. A novel antioxidant, stobadine, was found to reduce the increased vascular permeability and the extent of small intestine injury caused by I/R, to improve biochemical alterations accompanying I/R, to protect endothelial-dependent relaxation of mesentric arteries, and to attenuate the CL response. The obsereved beneficial effect of stobadine indicates its possible application in the preventive and/or therapeutic approach to I/R-induced pathologies. (c) 2007 Prous Science. All rights reserved.