Diffuse large B-cell lymphomas with germinal center B-cell-like differentiation immunophenotypic profile are associated with high apoptotic index, high expression of the proapoptotic proteins bax, bak and bid and low expression of the antiapoptotic protein bcl-xl
Diffuse large B-cell lymphomas with germinal center B-cell-like differentiation immunophenotypic profile are associated with high apoptotic index, high expression of the proapoptotic proteins bax, bak and bid and low expression of the antiapoptotic protein bcl-xl
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DOI：
10.1038/modpathol.3800130
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发表时间：
2004-07-01
期刊：
影响因子：
7.5
通讯作者：
Kanavaros, P
中科院分区：
文献类型：
--
作者：
Bai, M;Skyrlas, A;Kanavaros, P
本研究的目的是分析弥漫性大b细胞淋巴瘤的分化免疫表型与细胞凋亡和增殖状况的关系。因此，我们研究了79例弥漫性大b细胞淋巴瘤的bc16/CD10/MUM1/CD138分化免疫表型与(a)凋亡指数，(b)凋亡相关的bc12家族蛋白bc12、bcl-xl、bax、bak、bad和bid， (c)增殖指数（Ki67）和(d)细胞周期蛋白cyclin a、cyclin B1、cyclin D3、cyclin E、p53、Rb、p16和p27的关系。两种主要的分化免疫表型谱被区分出来：生发中心b细胞样谱；31例（bc16+/CD10+/MUM1-/CD138-: 29例，bc16-/CD10+/MUM1-/ CD138-: 2例）和非末端中心b细胞样谱（bc16+/-/CD10-/MUM1 +/CD138-）；48例。生发中心b细胞样谱中bax、bak和bid的表达及凋亡指数显著高于非生发中心b细胞样谱（P值分别为0.045、0.018、0.003和0.034）。相比之下，bcl-xl在生发中心b细胞样谱中的表达明显低于非生发中心b细胞样谱（P = 0.026）。bc16、CD10的表达与bax （r = 0.659， P< 0.001, r = 0.240, P = 0.033）、bak （r = 0.391, P = 0.033）的表达呈显著正相关
The aim of this study was to analyze the relations between differentiation immunophenotypes and the status of apoptosis and proliferation in diffuse large B-cell lymphomas. Therefore, the bc16/CD10/MUM1/CD138 differentiation immunophenotypic profiles were studied in relation to (a) the apoptotic index, (b) the apoptosis-associated bc12 family proteins bc12, bcl-xl, bax, bak, bad and bid, (c) the proliferation index (Ki67) and (d) the cell cycle proteins cyclin A, cyclin B1, cyclin D3, cyclin E, p53, Rb, p16 and p27 in 79 cases of diffuse large B-cell lymphomas. Two major differentiation immunophenotypic profiles were distinguished: the germinal center B-cell-like profile; 31 cases (bc16+/CD10+/MUM1-/CD138-: 29 cases and bc16-/CD10+/MUM1-/ CD138-: two cases) and the nongerminal center B-cell-like profile (bc16+/-/CD10-/MUM1 +/CD138-); 48 cases. The expression of bax, bak and bid and the apoptotic index were significantly higher in the germinal center B-cell-like profile than in the nongerminal center B-cell-like profile (P = 0.045, 0.018, 0.003 and 0.034, respectively). In contrast, the expression of bcl-xl was significantly lower in the germinal center B-cell-like profile than in the nongerminal center B-cell-like profile (P = 0.026). The expression of bc16 and CD10 showed significant positive correlation with the expression of bax (r = 0.659, P< 0.001 and r = 0.240, P = 0.033, respectively), bak (r = 0.391, P