A novel porous cells scaffold made of polylactide-dextran blend by combining phase-separation and particle-leaching techniques
A novel porous cells scaffold made of polylactide-dextran blend by combining phase-separation and particle-leaching techniques
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DOI：
10.1016/s0142-9612(02)00168-0
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发表时间：
2002-12-01
期刊：
影响因子：
14
通讯作者：
Wang, SG
中科院分区：
文献类型：
--
作者：
Cai, Q;Yang, JA;Wang, SG
本研究将聚乳酸（PLA）与天然的可生物降解葡聚糖共混制备了一种可生物降解材料，并采用溶剂铸造和颗粒浸出技术制备了一种新型海绵状支架。为了通过简单的溶剂铸造法获得PLA和葡聚糖的均匀共混物，需要通过三甲基硅基（TMS）保护葡聚糖的羟基，使葡聚糖可溶于有机溶剂。苯是少数几种能很好溶解tms保护的葡聚糖（TMSD）的溶剂之一，但它不是PLA的良好溶剂。因此，当使用二氯仿（DCM）和苯（v/v = 6/4）的混合物时，可以得到PLA和TMSD的均匀混合溶液。通过该技术，在扫描电镜（SEM）下观察到PLA-葡聚糖共混膜甚至PLA膜都形成了微孔结构（孔径约为5-10 μ m）。将聚乳酸和TMSD溶解在DCM和苯的混合溶剂中，以盐为成孔剂制备支架，观察到大孔（孔径约100-200 mum）的细胞壁上形成微孔（孔径约5-10 mum）。这种微孔结构与薄膜或泡沫形成过程中发生的相分离密切相关，这主要是由于PLA和TMSD在苯中的溶解度不同，以及DCM和苯的蒸发速率不同。与PLA相比，在甲醇中去除TMS基团后，PLA-葡聚糖共混膜或泡沫的表面亲水性和整体亲水性显著提高，细胞在这些聚合物底物上的培养结果显示细胞的附着和增殖能力增强。(C) 2002 Elsevier Science Ltd.版权所有。
In this study, a kind of biodegradable material was developed by blending polylactide (PLA) with natural biodegradable dextran, and a novel sponge-like scaffold made of it was fabricated thereof using solvent-casting and particle-leaching technique. To obtain a uniform blend of PLA and dextran by simple solvent-casting method, hydroxyls of dextran should be protected via trimethylsilyl (TMS) groups to make dextran soluble in organic solvents. Benzene was found among the few solvents that could dissolve this TMS-protected dextran (TMSD) well, however, it was not a good solvent for PLA. Therefore, a homogeneous mixed solution of PLA and TMSD could be obtained when a mixture of dichloroform (DCM) and benzene (v/v = 6/4) was used. By this technique, PLA-dextran blend films and even PLA films were observed a microporous structure (pore size around 5-10 mum) formation throughout the films under scanning electron microscope (SEM). Scaffolds that were prepared by dissolving PLA and TMSD in mixed solvent of DCM and benzene and using salt as porogen, were observed the formation of micropores (pore size around 5-10 mum) in the cellular walls of macropores (pore size around 100-200 mum). This microporous structure was closely related to the phase separation occurring during films or foams formation, which was mainly due to the different solubility of PLA and TMSD in benzene, as well as the different evaporation rates of DCM and benzene. In comparison with PLA, the surface and bulk hydrophilicity of PLA-dextran blend films or foams were significantly improved after the TMS groups were removed in methanol, and the results of cell culture on these polymeric substrates exhibited an enhancement on cell attachment and proliferation. (C) 2002 Elsevier Science Ltd. All rights reserved.