Stimulation of DNA synthesis by rat plasma following in vivo treatment with three liver mitogens.
Stimulation of DNA synthesis by rat plasma following in vivo treatment with three liver mitogens.
复制标题
用三种肝脏有丝分裂原进行体内处理后，大鼠血浆刺激 DNA 合成。
DOI：
10.1016/0304-3835(92)90293-5
复制
发表时间：
1992
期刊：
影响因子：
9.7
通讯作者：
Columbano,A
中科院分区：
文献类型：
--
作者：
Coni,P;Pichiri-Coni,G;Ledda-Columbano,GM;Semple,E;Rajalakshmi,S;Rao,PM;Sarma,DS;Columbano,A
本研究探讨了两种不同类型的肝细胞增殖刺激，即代偿性再生和直接增殖对正常肝细胞和癌前分离肝细胞DNA合成的影响。用硝酸铅(LN)、乙酸环丙孕酮(CPA)和二溴乙烷(EDB)三种不同的促肝原体物处理雄性Wistar大鼠，观察其对正常肝细胞和癌前肝细胞DNA合成的刺激作用。在CPA、EDB和PH给药后30min即可检测到DNA合成的诱导，并持续到给药后5d。此外，从癌前肝结节分离的肝细胞在培养中也能对这些因子诱导的DNA合成刺激产生反应。
The present study was undertaken to determine the effect of two different types of liver cell proliferative stimuli, namely compensatory regeneration and direct hyperplasia on DNA synthesis of normal and preneoplastic isolated hepatocytes. Platelet-poor plasma (PPP) isolated from male Wistar rats treated with three different hepatomitogens, lead nitrate (LN), cyproterone acetate (CPA) and ethylene dibromide (EDB), or subjected to surgical partial hepatectomy (PH), was tested for its ability to stimulate DNA synthesis in normal and preneoplastic hepatocytes in primary cultures. Induction of DNA synthesis was detected as early as 30 min after CPA, EDB and PH administration and persisted up to 5 days after the LN administration. In addition, hepatocytes isolated from preneoplastic liver nodules were also able to respond in culture to the DNA synthesis stimulus induced by these factors.