The modes of action of epsilon-polylysine (ε-PL) against Botrytis cinerea in jujube fruit
The modes of action of epsilon-polylysine (ε-PL) against Botrytis cinerea in jujube fruit
复制标题
ε-聚赖氨酸（ε-PL）对枣果灰葡萄孢的作用方式
DOI：
10.1016/j.postharvbio.2018.08.009
复制
发表时间：
2019-01-01
影响因子：
7
通讯作者：
Tian, Shiping
中科院分区：
文献类型：
--
作者：
Li, Hua;He, Chang;Tian, Shiping
ε-聚赖氨酸（epsilon-PL）是一种天然抗菌剂，已被广泛用作食品防腐剂。以往的研究表明，epsilon-PL是有效的控制食源性细菌，但很少有人知道它对采后病原真菌的控制效果和可能的作用方式。结果表明，epsilon-PL能显著抑制灰葡萄孢的菌丝生长、孢子萌发和芽管伸长，并能有效防治枣灰霉病。进一步研究表明，epsilon-PL对B.结果表明，epsilon-PL处理对枣果实的影响主要包括：（i）刺激胞内活性氧（ROS）的积累，降低病原菌致病相关基因的表达;（ii）导致可溶性碳水化合物和核酸的渗漏，破坏细胞质膜的完整性;（iii）诱导呼吸爆发氧化酶同源基因（RBOH）的表达。
Epsilon-polylysine (epsilon-PL) is a natural antimicrobial and has been widely used as a food preservative. Previous studies showed epsilon-PL being effective for control of foodborne bacteria; however, little is known about its control effects on postharvest pathogenic fungi and the possible modes of action. Here, we found that epsilon-PL could significantly inhibit mycelial growth, spore germination and germ tube elongation of Botrytis cinerea in vitro, as well as effectively control gray mold in harvested jujube fruit. Further investigation indicates that the modes of action of epsilon-PL against B. cinerea include (i) stimulating the accumulation of intracellular reactive oxygen species (ROS) and reducing the expression of pathogenesis-related genes of the pathogen, (ii) resulting in the leakage of soluble carbohydrates and nucleic acids, as well as the damage of plasma membrane integrity of the fungal cells, (iii) inducing the expression of respiratory burst oxidase homolog (RBOH) genes in epsilon-PL-treated jujube fruit.