Characterization of side-population cells in human normal endometrium
Characterization of side-population cells in human normal endometrium
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DOI：
10.1093/humrep/del514
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发表时间：
2007-05-01
影响因子：
6.1
通讯作者：
Wake, Norio
中科院分区：
文献类型：
--
作者：
Kato, Kiyoko;Yoshimoto, Momoko;Wake, Norio
背景技术背景：有人提出，人类子宫内膜可能含有一群成体干细胞，这些干细胞负责其显着的再生能力。最近，成人组织中的干细胞或祖细胞的子集已被鉴定为侧群细胞（SP细胞），其通过荧光激活细胞分选仪（FACS）分析显示Hoechst 33342的低染色。在这项研究中，我们分离SP细胞从人子宫内膜和分析其性质。实验方法：使用酶消化从子宫切除术中获得子宫内膜细胞，用于治疗子宫肌瘤，并用Hoechst 33342染料单独或与维拉帕米组合染色。然后使用FACS分析细胞。结果：SP细胞存在于正常人子宫内膜细胞中。大多数SP细胞富集在CD 9（-）CD 13（-）组分中。这些SP细胞显示出长期的再增殖特性，并产生腺（CD 9（+））和基质（CD 13（+））样细胞。从子宫内膜分离的CD 9-CD 13-细胞也产生腺样或基质样细胞。结论：人子宫内膜SP细胞具有前体细胞的功能。这是首次报道正常人子宫内膜SP细胞的表型。
BACKGROUND: It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties. METHOD: Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS. RESULTS: SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9(-)CD13(-) fraction. These SP cells showed long-term repopulating properties and produced gland (CD9(+))- and stroma (CD13(+))-like cells. CD9-CD13- cells isolated from the endometrium also generated gland- or stroma-like cells. CONCLUSIONS: SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells.