Tests of a mechanistic model of one hormone regulating both sexes in Cucumis sativus (Cucurbitaceae)
Tests of a mechanistic model of one hormone regulating both sexes in Cucumis sativus (Cucurbitaceae)
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DOI：
10.1002/j.1537-2197.1995.tb13856.x
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发表时间：
1995-12
影响因子：
3
通讯作者：
Tongjia Yin;J. A. Quinn
中科院分区：
文献类型：
--
作者：
Tongjia Yin;J. A. Quinn
一种开花植物性别决定的机制模型，假设一种激素有雄性和雌性细胞受体，独立地抑制一种性别并诱导另一种性别，用激素及其抑制剂在黄瓜(Cucumis Sativus)上进行了试验。赤霉酸(GA)及其合成抑制剂多效唑的应用表明，GA对性表达具有促进雄性和抑制雌性双重作用。相反，乙烯释放剂乙三醇和乙烯作用抑制剂硝酸银的应用表明，乙烯诱导雌雄并抑制雄化。这两种激素及其抑制剂的不同组合使用结果表明，乙烯对黄瓜GA具有压倒效应，并更直接地影响黄瓜的性别表达。这些实验表明，只有一种激素，而不是两种激素，调节黄瓜的性别表达，性激素很可能是乙烯。因此，结果支持了模型的假设，即一种激素可以通过单独诱导一种激素和抑制另一种激素来调节两性。模型预测证实，在雌雄同株系中，提高乙烯水平可以诱导雌株，降低乙烯水平可以诱导雄株。另一方面，正如模型预测的那样，乙烯或其抑制剂的应用诱导了两性株系的雌雄同株、雌株和三株植株。总之，单激素模型的所有假设和预测都在实验中得到了证实。
A mechanistic model of sex determination in flowering plants, which assumes that one hormone has male and female cell receptors to inhibit one sex and induce the other independently, was tested in cucumbers (Cucumis sativus) by applications of hormones and their inhibitors. Applications of gibberellic acid (GA) and an inhibitor of its synthesis (paclobutrazol) showed that GA had dual effects on sex expression of promoting maleness and inhibiting femaleness. Conversely, applications of Ethrel (an ethylene release agent) and AgNO3 (an ethylene action inhibitor) indicated that ethylene induced femaleness and inhibited maleness. Results of various combined applications of the two hormones and their inhibitors suggested that ethylene had overriding effects on GA and acted more directly on sex expression in cucumber. These experiments indicated that there is only one hormone, not two, regulating sex expression in cucumber, and that the sex hormone is likely to be ethylene. Results thus supported the assumption of the model that one hormone can regulate both sexes by inducing one and inhibiting the other independently. Model predictions were confirmed that from a monoecious line, female plants could be induced by increasing the ethylene level, and male plants induced by decreasing the ethylene level. On the other hand, as the model predicts, applications of ethylene or its inhibitor induced gynomonoecious, female, and trimonoecious plants from a hermaphroditic line. In conclusion, all assumptions and predictions of the one-hormone model were confirmed in the experiments.