Tissue distribution of the P2X(7) receptor
Tissue distribution of the P2X(7) receptor
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DOI：
10.1016/s0028-3908(97)00140-8
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发表时间：
1997-09-01
影响因子：
4.7
通讯作者：
Buell, G
中科院分区：
文献类型：
--
作者：
Collo, G;Neidhart, S;Buell, G
P2X(7)受体是一种双功能分子。三磷酸腺苷的结合可以在几毫秒内打开一条对小阳离子有选择性的通道，并在几秒钟内打开一个较大的孔，允许大到丙基染料(629 Da)的分子渗透。使用地高辛标记的核探针进行原位杂交，并使用针对C端肽序列的抗体进行免疫组织化学，以确定P2X(7)受体在大鼠和小鼠组织和细胞系中的分布。Northern blotting显示新生大鼠的大脑有一个6kb的RNA，但在成年大鼠的大脑中检测不到这种RNA。原位杂交和免疫组织化学显示，新生和成人脑内室管膜细胞均有大量标记，而脑实质未见标记。然而，P2X(7)受体免疫反应细胞出现在先前大脑中动脉闭塞引起的坏死区周围的半影区，表明该受体是由激活的小胶质细胞表达的。小鼠小胶质细胞系NTW8细胞强表达P2X(7)受体基因和蛋白。在大多数骨髓细胞中也观察到了P2X(7)受体的mRNA和蛋白，包括那些通过表达其他抗原如粒细胞、单核/巨噬细胞和B淋巴细胞而分别被鉴定的细胞。由脑巨噬细胞而不是神经元表达的P2X(7)受体在炎症、梗塞或免疫损伤后的大脑修复中所起的作用是一致的。(C)1997年爱思唯尔科学有限公司。
The P2X(7) receptor is a bifunctional molecule. The binding of ATP induces within milliseconds the opening of a channel selective for small cations, and within seconds a larger pore opens which allows permeation by molecules as large as propidium dyes (629 Da). In situ hybridization using a digoxigenin-labelled riboprobe, and immunohistochemistry using an antibody raised against a C-terminal peptide sequence, were used to determine the distribution of the P2X(7) receptor mRNA and protein in rat and mouse tissues and cell lines. The brain of newborn rats showed a 6 kb RNA by Northern blotting, but this was not detectable in adult brain. By in situ hybridization and immunohistochemistry, there was heavy labelling of ependymal cells in both newborn and adult brain, but the brain parenchyma showed no labelling. However, P2X(7) receptor-immunoreactive cells appeared in the penumbral region around an area of necrosis evoked by prior occlusion of the middle cerebral artery, suggesting expression of the receptor by activated microglia. NTW8 cells, a mouse microglial cell Line, strongly expressed the P2X(7) receptor mRNA and protein. The P2X(7) receptor mRNA and protein were also observed in the majority of bone marrow cells, including those separately identified by their expression of other antigens as granulocytes, monocyte/macrophages and B lymphocytes. The expression of P2X(7) receptor by brain macrophages rather than neurons would be consistent with a role in brain repair following inflammation, infarction or immune insult. (C) 1997 Elsevier Science Ltd.