Exploring potential bacterial and fungal biocontrol agents transmitted from seeds to sprouts of wheat
Exploring potential bacterial and fungal biocontrol agents transmitted from seeds to sprouts of wheat
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DOI：
10.1016/j.biocontrol.2016.02.013
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发表时间：
2016-07-01
影响因子：
4.2
通讯作者：
Cao, Lixiang
中科院分区：
文献类型：
--
作者：
Huang, Yali;Kuang, Zaoyuan;Cao, Lixiang
种传内生菌可垂直世代传播。这种垂直传播应选择对致病性和有利于互利共生，因为这些内生菌完全依赖于其宿主的生存和繁殖。为了阐明小麦种子萌发过程中垂直传播的核心细菌组和真菌组，通过基于Illumina的测序分析了芽、来自灭菌种子的芽和健康小麦植物的种子的细菌和真菌分类群。结果表明，灭菌种子的芽中含有的真菌比种子和芽中的真菌种类多，而种子中含有的细菌比芽中的细菌种类多。小麦种子和芽中含有不同的优势细菌和真菌类群。在种子和芽苗样品中均发现了属于欧文氏菌属和根瘤菌目的细菌OTU，但在芽苗中欧文氏菌的丰度有所下降。在种子和所有发芽样品中检测到优势真菌属Emericella。结果表明，小麦种子和芽中可能含有核心细菌组和真菌组，并可在小麦植株组织中传播。核心内生菌与寄主植物的相关性比其他微生物类群更密切，这一结果有助于阐明植物-微生物间的相互作用，并为小麦生产中选择合适的微生物生防制剂提供参考。(C)2016 Elsevier Inc. All rights reserved.
Seed-borne endophytes could be transmitted vertically from generation to generation. This vertical transmission should select against pathogenicity and favour mutualism as these endophytes depend entirely on their host for survival and reproduction. To elucidate the vertically transmitted core bacteriome and mycobiome throughout the germination process of wheat seeds, the bacterial and fungal taxa of sprouts, sprouts from sterilized seeds and seeds of healthy wheat plants were analyzed by Illumina-based sequencing. Results suggested that the sprouts from sterilized seeds contained more diverse fungi than those in the seeds and sprouts, nevertheless, the seeds contained more diverse bacteria than those in sprout samples. The wheat seeds and sprouts contained different dominant bacterial and fungal taxa. Bacterial OTUs belong to Erwinia and Rhizobiales were founded in seed and all sprout samples, but the abundance of Erwinia decreased in sprouts. The dominant fungal genus Emericella was detected in seed and all sprout samples. The results suggested that wheat seeds and sprouts might contain core bacteriome and mycobiome during the development from seeds to sprouts and could transmit in the plant tissues of wheat. The core endophytes should illustrate more intimate correlation with host plants than other microbial taxa and the results are helpful for elucidating the plant-microbe interactions and selecting suitable microbial biocontrol agents for wheat production. (C) 2016 Elsevier Inc. All rights reserved.