Notch signaling in astrocytes and neuroblasts of the adult subventricular zone in health and after cortical injury
Notch signaling in astrocytes and neuroblasts of the adult subventricular zone in health and after cortical injury
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DOI：
10.1159/000090755
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Bongarzone, ER
中科院分区：
文献类型：
--
作者：
Givogri, MI;de Planell, M;Bongarzone, ER
出生后心室下区（SVZ）是成人大脑中持续神经发生的一个生态位，可能在神经退行性疾病的自我修复反应中起着重要作用。该区域内神经干细胞池的维持依赖于细胞间的通讯，例如由Notch信号通路提供的通讯。Notch1受体mRNA被发现分布在出生后大脑的不同区域，包括SVZ。尽管在这些区域中的大多数已经确定了表达notch1的细胞的身份，但尚不清楚SVZ内哪些细胞类型表达该途径的成分。在成人SVZ中，Notch1的大部分表达发生在多唾液化神经细胞粘附分子（PSA-NCAM）阳性的神经前体和胶质纤维酸性蛋白阳性的SVZ星形胶质细胞中。Notch1也存在于吻侧迁移流（RMS）中psa - ncam阳性的神经母细胞中，但在到达嗅球的神经母细胞中较少。我们发现两种天然存在的Notch1激活因子Jagged1和Delta1也在成年小鼠大脑的SVZ和RMS中表达。最后，通过皮质刺伤模型，我们发现SVZ对损伤的星形胶质源性反应伴随着Notch通路的激活。
The postnatal subventricular zone (SVZ) is a niche for continuous neurogenesis in the adult brain and likely plays a fundamental role in self-repair responses in neurodegenerative conditions. Maintenance of the pool of neural stem cells within this area depends on cell-cell communication such as that provided by the Notch signaling pathway. Notch1 receptor mRNA has been found distributed in different areas of the postnatal brain including the SVZ. Although the identity of Notch1-expressing cells has been established in the majority of these areas, it is still unclear what cell types within the SVZ are expressing components of this pathway. Here we demonstrate that most of expression of Notch1 in the adult SVZ occurs in polysialylated neural cell adhesion molecule (PSA-NCAM)-positive neural precursors and in glial fibrillary acidic protein-positive SVZ astrocytes. Notch1 was also found in PSA-NCAM-positive neuro-blasts located within the rostral migratory stream (RMS) but much less in those that have reached the olfactory bulb. We show that two of the naturally occurring Notch1 activators, Jagged1 and Delta1, are also expressed in the SVZ and within the RMS in the adult mouse brain. Finally, using a model of cortical stab wound, we show that the astrogliogenic response of the SVZ to injury is accompanied by activation of the Notch pathway.