LEUKOTRIENE-B-4 STIMULATES POLYMORPHONUCLEAR LEUKOCYTE ADHESION TO CULTURED VASCULAR ENDOTHELIAL-CELLS
LEUKOTRIENE-B-4 STIMULATES POLYMORPHONUCLEAR LEUKOCYTE ADHESION TO CULTURED VASCULAR ENDOTHELIAL-CELLS
复制标题
DOI：
10.1172/jci111570
复制
发表时间：
1984-01-01
影响因子：
15.9
通讯作者：
SCHAFER, AI
中科院分区：
文献类型：
--
作者：
GIMBRONE, MA;BROCK, AF;SCHAFER, AI
多形核白细胞（PMN）粘附于血管内皮细胞是炎症反应的重要组成部分。我们使用定量单层粘附试验研究了花生四烯酸的各种脂氧合酶代谢物对中性粒细胞与培养的血管内皮细胞粘附的影响。结果表明，白三烯B_4（LTB_4）能有效地促进中性粒细胞与内皮细胞表面的粘附，而硫肽白三烯C_4、D_4和E_4以及白细胞和血小板的单羟酸脂氧合酶产物5S-羟基-6-反式-8，11，14-顺式-二十碳四烯酸和12 S-羟基-5，8-顺式，10-反式，14-顺式-二十碳四烯酸则无此作用。LTB 4刺激中性粒细胞-内皮细胞粘附似乎不依赖于环氧合酶代谢产物的产生，也不受外源性前列环素的抑制。在几个物种的不同类型的大血管（动脉和静脉）培养的内皮细胞中观察到PMN粘附增强。这些发现表明LTB 4在调节白细胞-血管壁相互作用中具有重要的病理生理作用。
Adhesion of polymorphonuclear leukocytes (PMN) to the endothelial lining of blood vessels is an essential component of the inflammatory response. We have examined the effects of various lipoxygenase metabolites of arachidonic acid on PMN adhesion to cultured vascular endothelial cells, using a quantitative monolayer adhesion assay. Our results indicated that leukotriene B4 (LTB4) could effectively stimulate PMN adhesion to endothelial cell surfaces, in contrast to the sulfidopeptide leukotrienes C4, D4, and E4, and the monohydroxyacid lipoxygenase products of leukocytes and platelets, 5S-hydroxy-6-trans-8,11,14-cis-eicosatetraenoic acid and 12S-hydroxy-5,8-cis,10-trans,14-cis-eicosatetraenoic acid, respectively. LTB4-stimulation of PMN-endothelial adhesion did not appear to be dependent upon the generation of cyclooxygenase metabolites, nor was it inhibited by exogenous prostacyclin. Enhanced PMN adhesion was observed with endothelial cells that were cultured from different types of large vessels (arteries and veins) in several species. These findings suggest an important pathophysiologic role for LTB4 in regulating leukocyte-vessel wall interactions.