Detection of JC virus DNA sequences in brain tumors in pediatric patients
Detection of JC virus DNA sequences in brain tumors in pediatric patients
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DOI：
10.3171/ped.2005.102.3.0294
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发表时间：
2005-04-01
影响因子：
4.1
通讯作者：
Tabuchi, K
中科院分区：
文献类型：
--
作者：
Okamoto, H;Mineta, T;Tabuchi, K
Object. JC病毒是一种引起进行性多灶性白质脑病的人类嗜神经性多瘤病毒，与猿猴病毒40密切相关。最近的几份报告表明JC病毒与各种人类脑肿瘤的发生之间可能存在联系。作者检测了儿童患者原发性脑肿瘤中JC病毒DNA序列的存在情况，以评估儿童人群中特定脑肿瘤可能因JC病毒感染而发生的假设。从62个脑肿瘤（32个髓母细胞瘤，18个室管膜瘤，5个脉络丛乳头状瘤和7个毛细胞星形细胞瘤）中分离基因组DNA序列，并通过Southern印迹杂交和直接测序分析JC病毒DNA的存在。JC病毒DNA序列在5例室管膜瘤和1例脉络丛乳头状瘤中检出。免疫组化研究显示，脉络丛乳头状瘤的大T抗原的核表达。所有髓母细胞瘤和毛细胞型星形细胞瘤均不含JC病毒DNA。本研究的结果提供了JC病毒与某些室管膜瘤和脉络丛乳头状瘤的发展之间的关联的分子证据。
Object. The JC virus is a human neurotropic polyomavirus that causes progressive multifocal leukoencephalopathy and is closely related to simian virus 40. Several recent reports have indicated a possible association between the JC virus and the development of various human brain tumors. The authors examined the presence of JC virus DNA sequences in primary brain tumors in pediatric patients to evaluate the hypothesis that particular brain tumors can arise in the pediatric population as a consequence of infection with the JC virus.Methods. Genomic DNA sequences were isolated from 62 brain tumors (32 medulloblastomas, 18 ependymomas, five choroid plexus papillomas, and seven pilocytic astrocytomas) and analyzed for the presence of JC virus DNA by Southern blot hybridization and direct sequencing. The JC virus DNA sequence was detected in five ependymomas and one choroid plexus papilloma. Immunohistochemical studies revealed nuclear expression of the large T-antigen in a choroid plexus papilloma. None of the medulloblastomas or pilocytic astrocytomas contained JC virus DNA.Conclusions. The results of this study provide molecular evidence of the association between JC virus and the development of certain ependymomas and choroid plexus papillomas.