Stable microreentrant sources as a mechanism of atrial fibrillation in the isolated sheep heart
Stable microreentrant sources as a mechanism of atrial fibrillation in the isolated sheep heart
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DOI：
10.1161/01.cir.101.2.194
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发表时间：
2000-01-18
期刊：
影响因子：
37.8
通讯作者：
Jalife, J
中科院分区：
文献类型：
--
作者：
Mandapati, R;Skanes, A;Jalife, J
背景：心房颤动（AF）传统上被描述为非周期性或随机性。然而，高频周期性活动的持续来源最近被认为是羊心脏房颤的基础。我们的目的是结合光学和双极电极记录来确定AF期间周期性活动的位置并阐明其机制。方法与结果：在0.1 ~ 0.5 μ mol/L乙酰胆碱作用下，采用快速起搏法诱导7只兰根多夫羊心脏发生心房颤动。我们同时对左右心房（RA和LA）进行光学测绘，并对光学和双极电极记录（包括一个漫游电极）进行频率采样，以确定具有最高主导频率（DF）的位点。从光学记录中识别转子，并测量其旋转周期，核心面积和周长。总共分析了35例房颤发作。平均LA和RA df分别为14.7+/-3.8和10.3+/-2.1 Hz。在所有事件中，LA均表现出时空周期性。在7个实验中，有5个在最高DF处具有周期性活性的单个位点被定位。最高的DF最常（80%）定位于LA后部，靠近或位于肺静脉口。转子（n=14）定位在左旋肌上。平均核心周长和面积分别为10.4+/-2.8 mm和3.8+/-2.8 mm(2)。结论：频率采样可以快速识别AF期间高频周期性活动的离散位点。在该模型中，稳定的微重入源最有可能是AF的潜在机制。
Background-Atrial fibrillation (AF) has traditionally been described as aperiodic or random. Yet, ongoing sources of high-frequency periodic activity have recently been suggested to underlie AF in the sheep heart. Our objective was to use a combination of optical and bipolar electrode recordings to identify sites of periodic activity during AF and elucidate their mechanism.Methods and Results-AF was induced by rapid pacing in the presence of 0.1 to 0.5 mu mol/L acetylcholine in 7 Langendorff-perfused sheep hearts. We used simultaneous optical mapping of the right and left atria (RA and LA) and frequency sampling of optical and bipolar electrode recordings (including a roving electrode) to identify sites having the highest dominant frequency (DF). Rotors were identified from optical recordings, and their rotation period, core area, and perimeter were measured. In all, 35 AF episodes were analyzed. Mean LA and RA DFs were 14.7+/-3.8 and 10.3+/-2.1 Hz, respectively. Spatiotemporal periodicity was seen in the LA during all episodes. In 5 of 7 experiments, a single site having periodic activity at the highest DF was localized. The highest DF was most often (80%) localized to the posterior LA, near or at the pulmonary vein ostium. Rotors (n=14) were localized on the LA. The mean core perimeter and area were 10.4+/-2.8 mm and 3.8+/-2.8 mm(2), respectively.Conclusions-Frequency sampling allows rapid identification of discrete sites of high-frequency periodic activity during AF. Stable microreentrant sources are the most likely underlying mechanism of AF in this model.